摘要
本研究以番鸭肝脏为试验材料，通过RT-PCR扩增出番鸭脂肪酸合成酶（fatty acid synthase，FAS）部分CDS序列，利用相关分子生物学软件对其进行分析，同时采用实时荧光定量PCR技术检测FAS基因在成年番鸭各组织中的表达特性及填饲对番鸭肝脏和腹脂中该基因表达的影响。结果表明，克隆出的番鸭FAS基因CDS片段大小为1122 bp，编码374个氨基酸，与近缘物种禽类核苷酸同源性为92%～99%；实时荧光定量PCR结果表明，番鸭FAS基因在肝脏和腹脂中高表达，说明FAS具有组织表达特异性；填饲导致肝脏中FAS基因表达显著升高（P＜0.05）。本试验结果可为进一步研究FAS基因在番鸭肝脏脂质代谢中的作用奠定基础。
The Muscovy duck were used to clone the sequence of fatty acid synthase （FAS） gene by RT-PCR，the sequence was analyzed by related softwares and on-line tools, the tissue-specific expression and the effect of overfeeding on FAS gene mRNA level were performed by Real-time PCR. The results showed that the cloned sequence was 1122 bp and encoded 374 amino acids. The nucleotide homology of Muscovy duck FAS was high similar with other avain species from 92% to 99%. The tissue-specific expression result showed that the mRNA expression level of Muscovy duck FAS gene was the higher in liver and adipose tissues and was not detected or little in other 10 tissues. It revealed that Muscovy duck FAS gene was tissue specific.The mRNA expression of Muscovy duck FAS gene in liver was significantly increased after overfeeding （P〈0.05）.The results indicated that FAS gene could play an important role in duck liver steatosis.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第1期84-87,共4页
China Animal Husbandry & Veterinary Medicine
基金
江苏省高校优势学科建设工程资助
江苏省"333工程"(苏人才[2011]15号)
江苏省"青蓝工程"资助(苏教师[2012]39号)
关键词
番鸭
脂肪酸合成酶
基因克隆
填饲
Muscovy duck
fatty acid synthase
gene clone
overfeeding
