摘要
目的研究大豆苷元对脑缺血再灌注损伤的保护作用,并初步探讨其保护作用与炎症反应之间的关系。方法采用大鼠大脑中动脉栓塞制作局灶性脑缺血再灌注损伤模型,缺血后10 min,于背部皮下注射不同剂量的大豆苷元。缺血2h,再灌注24 h后,断头取脑,TTC染色,测定脑梗死体积;取前脑称湿重,烘干后称干重,计算脑组织含水率;取血2 ml,分离血清,放射免疫学方法检测血清IL-1β及TNF-α含量。结果与模型组相比,大豆苷元治疗组脑梗死范围减小,脑组织含水率降低(P<0.05),血清IL-1β及TNF-α含量(P<0.05)降低。结论大豆苷元对脑缺血再灌注损伤具有一定的保护作用,其保护机制与降低炎性细胞因子TNF-α、IL-1β的表达进而减轻脑水肿有关。
Objective To explore the protective effects of Daidzein（DD） on cerebral ischemia- reperfusion injury and study it~ mechanism about inflammatory response and cytokine secretion. Methods A rat ischemia - reperfusion injury animal model was made through embolization of the middle cerebral artery. The injury rats were treated with DD, using sublingual vein injection. The volumes of infarction and water content in the cerebral tissue, and the levels of IL - 113 and TNF - c~ in the serum were meas- ured. Results After treatment, the rats with cerebral ischemia - reperfusion injury showed a decrease in the volumes of infarction, water content（P 〈 0.05 ） , IL- 113 and TNF -a（P 〈 0.05）. Conclusion DD has a therapeutic effect on rat cerebral ischemia- reperfusion injury because it down - regulates IL - 113 and TNF expression, inhibits inflammatory response, and then decreases cerebral edema.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2014年第1期6-8,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30760284
No.81160399)
关键词
大豆苷元
脑缺血再灌注损伤
脑水肿
细胞因子
Daidzein
Cerebral ischemia - reperfusion injury
Cerebral edema
Cytokine
