摘要
目的:探讨二氢卟吩e6(Ce6)光动力学疗法(Ce6-PDT)对体外培养人舌鳞癌Tca8113细胞的杀伤作用。方法:向体外培养的人舌鳞癌Tca8113细胞中加入Ce6,浓度分别为5、10、20、50和100μg/ml,孵育1.5h,予波长630 nm半导体激光照射,照射的功率密度为100mW/cm2,能量密度分别为0.5、1、2、5、10和15J/cm2,继续孵育6h,用MTS比色法测定细胞存活率。结果:(1)不受光照的条件下,较低浓度Ce6(<10μg/ml)与细胞共同培养而对细胞的毒性较小,Ce6浓度为20μg/ml时对细胞的杀伤率约为10%。(2)光照条件下,Ce6-PDT能有效地杀伤体外培养的人舌鳞癌Tca8113细胞,其杀伤作用与Ce6浓度及光照剂量正相关,呈浓度/剂量依赖性(P<0.01)。结论:Ce62PDT能有效地杀伤体外培养的人舌鳞癌Tca8113细胞;Ce6在较大浓度下对细胞有毒性。
Objective： To investigate the photodynamic effects of chlorine e6 （Ce6） on the killing ability to human Tongue Squamous Cell Carcinoma Tca8113 cells in vitro. Methods： Cultured human Tongue Squamous Cell Carcinoma Tca8113 cells were incubated in a medium containing various concentrations（5,10,20,50 和 100μg/ml）of chlorin e6 for 1.5 h and illuminated with different light doses （0.5, 1, 2, 5, 10 and 15J/cm2） using a semiconductor laser （wavelength 630nm; energy density of 100mW/cm2）. 12h after the Ce6-PDT,the survival rate of human Squamous Cell Carcinoma Tca8113 cells was analyzed by MTS assay. Results： （1） The cytotoxicity of Ce6 on human squamous cell carcinoma Tca8113 cells was little while there was no irradiation and the concentrations of chlorin e6 were less than 10μg/ml （P〉0.05）. But if the concentrations of chlorin e6 were more than 20μg/ml, even if there were no irradiation, it could affect the livability of human squamous cell carcinoma Tca8113 cells （P〈0.05）. （2） Ce6-PDT can kill human squamous cell carcinoma Tca8113 cells effectively in vitro.Higher phototoxicity was obtained with higher dye and /or laser doses.Among the various concentrations of chlorin e6 and laser doses, the viability of human squamous cell carcinoma Tca8113 cells varies in a dose-dependent mariner（P〈0.01）. Conclusions： Ce6-PDT can kill the human squamous cell carcinoma Tea8113 cells effectively in vitro. When in high concentrations, Ce6 can induce dark cytotoxicity on human squamous cell carcinoma Tca8113 cells.
出处
《中华老年口腔医学杂志》
2013年第4期205-208,253,共5页
Chinese Journal of Geriatric Dentistry
基金
北京市自然科学基金(编号:7122174)
