摘要
对氧化葡萄糖酸杆菌 (Gluconobacteroxydans)SCB32 9的纯培养进行了研究 ,测定了其生长曲线 ,确定其对数期为 4～ 2 7h。获得纯培养的对数期菌体后采用凝胶包埋法制备完整染色体 ,用脉冲场电泳方法对SCB32 9的染色体进行了分析 ,确定其有一条染色体和一个大质粒。染色体的长度在 2 2Mb到 3 5Mb之间。
After the pure culture of Gluconobacter oxydans SCB329 was researched,its growth curve was measured and its logarithmic phase was determined as between 4\|27h.After the microorganisms were havested in its logarithmic phase, The intact chromosome was prepared by agarose\|embedded method.Then the genome of SCB329 was analyzed by Pulsed\|field Gel Electrophoresis.The result indicated that there are one chromosome and one great plasmid.The length of intact chromosome of SCB329 has been estimated to be approximately between 2.2Mb and 3.5Mb.
出处
《微生物学报》
CAS
CSCD
北大核心
2001年第1期40-53,共14页
Acta Microbiologica Sinica
基金
国家自然科学基金资助项目!( 39970 0 2 4 )
河北维生药业公司科研合作经费资助&&
关键词
氧化葡萄糖酸杆菌
纯培养
凝胶包埋法
脉冲电泳
染色体
发酵
维生素C
Gluconobacter oxydans , Pure Culture, Agarose\|embedded method, Pulsed\|field gel electrophoresis, Chromosome
