摘要
目的观察镰形棘豆生物碱提取物对9种常见致病菌的抗菌作用。方法以镰形棘豆为原料，提取生物碱，选择临床常见的4种革兰氏阳性菌和5种革兰氏阴性菌，采用琼脂扩散法对其体外抑菌活性进行评价，试管二倍稀释法测定其最低抑菌浓度（MIC）和最小杀菌浓度（MBC）。结果镰形棘豆生物碱提取物对9个菌种均有抑菌效果，抑菌作用敏感度与浓度呈良好的量效关系，其中对枯草杆菌和嗜麦芽窄食单胞菌的抑菌效果优于其他各菌，MIC和MBC分别为0．95mg／mL和1．91mg／mL，而其他各菌种的MIC和船C范围在1．91～7．63mg／mL之间。结论镰形棘豆生物碱提取物具有较为广谱的抑菌作用，对革兰氏阳性菌的抑制作用比革兰氏阴性菌强。
Objective To investigate the antibacterial activity of the alkaloids extract from Oxytropis falcata Bunge against 9 common pathogens in vitro. Methods Alkaloids was extracted from Oxytropis faleata Bunge. The agar plate dilution method was adopted to evaluate the activity against bacteria of 4 Gram-negative test strains and 5 Gram-positive test strains, while the MIC and MBC were determined by tube double dilution method. Results The alkaloids extract from Oxytropis falcata Bunge had the antibacterial activity against 9 pathogens and there was a good dose-response relationship between sensitivity and concentration. Antibacterial activities against bacillus subtilis and stentrophmomas maltophilia showed stronger than other tested bacteria, the MIC and MBC were 0.95 mg/mL and 1.91 mg/mL, respectively. In other tested bacteria, MIC and MBC were ranged in 1.91-7.63 mg/mL. Conclusion The alkaloids extract showed a broad spectrum of a stronger antibacterial activity against all tested pathogens. Gram-positive strains were more sensitive than Gram-negative strains to the inhibitory effects of the alkaloids extract.
出处
《中国中医药信息杂志》
CAS
CSCD
2014年第2期53-55,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81260633)
关键词
镰形棘豆
生物碱
致病菌
抑菌作用
Oxytropis falcata Bunge
alkaloids extract
pathogens
antibacterial activity
