摘要
cDNA微阵列已在基因差异表达、寻找新基因等研究方面获得广泛应用 ,但有关cDNA微阵列的制作 ,目前多采用多聚赖氨酸修饰的载玻片为探针固定载体 ,固定效果较差 .用氨基硅烷处理的载玻片为载体制作cDNA微阵列 ,然后考察其固定效率、检测灵敏度、稳定性、实用性等指标 .结果表明 ,用氨基硅烷处理的载玻片具有比多聚赖氨酸更令人满意的核酸固定效率、检测灵敏度 ,且稳定实用 .因此 ,用氨基硅烷修饰的载玻片为探针固定载体制作cDNA微阵列较为理想 .
The use of microarrays of oligonucleotides or cDNA is considered to be a promising approach for DNA and RNA sequence analysis, diagnostics of generic diseases, gene polymorphism studies and analysis of gene expression. To manufacture cDNA microarrays the samples were printed onto glass microscope slides treated with poly-L-lysine, and then the slides were processed by heat and UV light treatment to attach the cDNA sequence to the glass surface. But the immobilization efficiency of cDNA on the glass surface was low. A simple procedure for manufacture cDNA microarrays on a slide treated with 3-aminopropyltrimethoxysiiane is described. The efficiency for attaching cDNA to the amino-modified slides is greater than that to the slides treated with poly-L-iysine. The cDNA microarray made by the amino-modified slides is stable for use in 80 degreesC, 75% humidity, 3 600Lx light, exposure in air, respectively.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第1期121-124,共4页
Progress In Biochemistry and Biophysics
基金
中国科学院九五特别支持项目! (KY95T 12 )&&
