摘要
通过将凝血酶抑制剂水蛭素的C端 2 0肽片段嫁接到血小板结合蛋白AnnexinⅤ上 ,可以期望获得既具有抗凝血活性 ,同时又具有导向性的新型工程蛋白质分子 .利用计算机辅助分子设计手段模拟了该融合蛋白的分子结构 ,并对该融合蛋白对凝血酶的抑制作用进行了分子动力学模拟 ,得到了支持上述想法的结果 .
Hirudin is one of the most potent anti-coagulant protein ever found, and its C-terminus is a key domain for inhibiting thrombin. In order to enhance its specificity, a novel anti-coagulant protein was constructed via fusing the C-terminus of hirudin to Annexin V, which was expected to sustain both anticoagulant activity and phorspholipid affinity. The structure of the designed protein was predicted with both molecular mechanics and dynamics. Molecular dynamics was adopted to simulate the docking interaction between the fusion protein and thrombin. The results showed the inhibitory activity of the fusion protein to thrombin.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第1期86-89,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金! (39970 184
79970 116 )
霍英东青年教师奖励基金
江苏省自然科学基金! (BJ970 41)资助项目&&
