摘要
报道了一种鱼类二价体显带的新方法。采用 pH值在 7 2～ 7 4之间的低渗液对大鳞副泥鳅精巢进行整体长低渗、卡诺固定液处理方法得到了粗线期二价染色体的高分辨G带 ,其带纹特征和数目相对稳定且清晰可辨。与胰酶显带法和EDTA显带法相比具有操作简单、耗时短以及二价体分散相对较好等特点。这一方法在所有显带方法中最为简便。
A new method for Pachytene chromosome G banding was reported in this paper.The whole testis was treated with basic hypotonic solution(pH 7.2～7.4) for 4～6 h,then treated with Carnoy's solution for 3～4 times.The cell suspensions were discarded on cold slides.The high resolution G bands of meiotic pachytene chromosome in Paramisgurnus dabryanus was obtained after dyed with Giemsa.The characteristic and numbers of bands were stable and distinct.Compared with trypsin or EDTA treated methods,this protocol is the simplest one with operation simple,less time needed and high rate of success.
出处
《动物学杂志》
CAS
CSCD
北大核心
2001年第1期23-26,共4页
Chinese Journal of Zoology
基金
国家自然科学基金! (No.39770 40 7)资助
关键词
大鳞副泥鳅
粗线期染色体
碱性低渗液显带法
分带研究
Paramisgurnus dabryanus
Pachytene chromosome
Banding method with basic hypotonic solution
