摘要
目的探讨莪术油对人乳头状瘤病毒(human papillomavirus,HPV)16亚型的抑制作用。方法以不同浓度的含莪术油培养液体外培养宫颈癌细胞系SiHa、CaSki和宫颈永生化细胞H8,相差显微镜下观察活细胞形态学变化;采用四甲基偶氮唑蓝比色法、流式细胞术检测不同浓度莪术油对细胞增殖的影响;应用反转录-聚合酶链反应技术(reverse transcriptase polymerase chain reaction,RT-PCR)检测人乳头状瘤病毒HPV16亚型E6E7的表达。结果不同浓度的莪术油可以抑制细胞的增殖;莪术油作用于SiHa细胞,G1期细胞减少(P<0.05),G2、S期细胞增加(P<0.01),细胞阻滞于G2、S期。莪术油作用于CaSki、H8细胞,G1期细胞减少(P<0.01),S期细胞增加(P<0.01),使细胞阻滞于S期。加药组SiHa、CaSki和H8细胞凋亡率高于对照组,有统计学差异(P<0.01,P<0.01,P<0.05);三种细胞HPV16E6E7基因片段mRNA表达均明显低于对照组(P<0.01)。结论莪术油在体外抑制宫颈癌细胞系SiHa、CaSki和宫颈永生化细胞H8增殖,其机制可能是通过抑制HPV16E6E7表达而抑制细胞生长。
Objective To study the inhibition of HPV16 by oleum curcumae. Methods The human cervical cancer cell line- CaSki, SiHa and the immortalized cervical epithelial cell-H8 were cultured with different concentrations of oleum cureumae. Changes in cellular form were observed by phase contrast microscopy. The proliferative inhibition under different concentrations of oleum curcumae on CaSki, SiHa and H8 was measured by methyl thiazolyl tetrazolium（MTF） assay and flow cytometry assay. The mRNA expression of HPV16 E6E7 was determined by RT-PCR semi-quantitatively. Results The growth of CaSki, SiHa and H8 was inhibited by different concentrations of oleum curcumae. SiHa cells were accumulated in G2, S phase（ P 〈 0.01 ） , while CaSki, H8 cells were accumulated in S phase （ P 〈 0.01 ）. The apoptosis rates in CaSki, SiHa and H8 cells were higher than those in control group（ P 〈 0.01 ,P 〈 0.01, P 〈 0.05）. After treated by oleum eurcumae, CaSki, SiHa and H8 cell showed decreased level of HPV16 E6E7 mRNA than that in the control group （ P 〈 0.01 ）. Conclusions Oleum curcumae may execute its antiHPV activity primarily by decreasing the expression of HPV 16E6E7 in cervical cancer cell line and immortalized cervical epithelial cells.
出处
《武警医学》
CAS
2014年第1期19-23,共5页
Medical Journal of the Chinese People's Armed Police Force
关键词
莪术油
人乳头状瘤病毒
反转录-聚合酶链反应
oleum curcumae
human papillomavirus
reverse transcriptase polymerase chain reaction
