摘要
对克雷伯氏杆菌代谢甘油产1,3-丙二醇的代谢网络进行了基元模式分析,并结合不同发酵阶段下的胞外代谢产物积累速率,以生物量最大化为目标函数,应用线性规划对其微氧和厌氧条件下的代谢网络进行优化分析,得出不同发酵阶段各基元模式的通量值。结果表明:微氧条件下不同发酵阶段生物量合成的最优基元模式相同;厌氧条件下延迟期和稳定期的最优基元模式相同,对数期的最优基元模式不同;各最优基元模式对1,3-丙二醇合成贡献较大;氧化磷酸化途径在维持NAD+/NADH2和ATP/ADP平衡中起重要作用,更有利于生物量和1,3-丙二醇的合成。
The metabolic network of Klebsiella pneumoniae with glycerol for 1,3-propanediol production was analyzed with elementary modes analysis. The methodology in conjunction with experimentally accumula- tion rates of external metabolites for K. pneumoniae at different phases of fermentation was used for optimizing and analyzing the metabolic network under micro-aerobic and anaerobic conditions. The values of elementary mode fluxes at different phases of fermentation were obtained by linear programming with maximization of bio- mass as the objective function. The results showed that the optimum elementary modes contributing biomass synthesis were identical at different phases of fermentation under micro-aerobic condition and the optimum ele- mentary modes of delay period and stable period were identical and the different optimum elementary modes were obtained at logarithm period under anaerobic condition. In addition, it was found that the optimum elemen- tary modes contributing biomass synthesis were propitious to 1,3-propanediol production. The oxidative phos- phorylation pathway played an important role on maintaining NAD＋/NADH2 and ATP/ADP balance and the synthesis of biomass and 1,3-propanediol.
出处
《化学与生物工程》
CAS
2014年第2期30-34,共5页
Chemistry & Bioengineering
基金
山东省自然科学基金资助项目(ZR2013BM001)
关键词
基元模式分析
克雷伯氏杆菌
代谢网络
生物量最大化
1
3-丙二醇
elementary modes analysis
Klebsiella pneumoniae
metabolic network
maximization of biomass
1,3-propanediol
