摘要
目的 探讨一氧化氮 (NO)在胃食管反流病 (GERD)发病机制中的作用。方法 应用PCpolygrafHR高分辨多通道测压系统检测GERD患者的食管下段括约肌压力 (LESP)、食管下段括约肌长度 (LESL)及食管远端蠕动幅度等动力参数 ;应用Digitrap perMKⅢ动态食管PH监测仪检测其 2 4小时食管内PH各项参数 ;应用NADPH -d组化染色观察食管一氧化氮合酶 (NOS)表达 ;应用硝酸还原酶法测定血清NO含量。结果 GERD患者的LESP及LESL显著低于对照组 (P <0 0 1) ;前者的食管下段蠕动幅度明显低于后者 (P <0 0 1) ;前者的食管内 2 4小时PH值明显高于后者 ;患者食管粘膜NOS呈强阳性反应 ;其血清NO含量也显著高于对照组。结论 内源性NO可能参与GERD的致病机理。
Objective To explore the effect of nitric oxide (NO) on pathogenesis in patients with gastroesophageal reflux disease(GERD).Methods Parameters included LES pressures(LESP),LES length (LESL),the distal esophageal peristaltic amplitude were determined using PC polygraf HR manometry testing system;24-hour PH were detected by Digitrapper MK Ⅲ ambulant monitor;The expression of NO was observed using NADPH-diaphorase (NADPH-d) histochemical staining;In addition,the concentration of NO in serum was also measured.Results LESP,LESL in patients with GERD were lower than that in controls(P<0.01);The peristaltic amplitude of lower esophagus in former was lower than that in latter;24-hour PH-metry in patients with GERD were higher than that in normal controls;Intensity of NOS staining in the esophageal epithelium of patients with reflux esophagitis was significantly stronger than that of controls;Serum NO concentration in patients with reflux esophagitis was significantly higher in comparison with that of controls.Conclusion The endogenous nitric oxide may play an important role in pathogenesis of gastroesophageal reflux disease.
出处
《胃肠病学和肝病学杂志》
CAS
2000年第4期273-275,共3页
Chinese Journal of Gastroenterology and Hepatology
