摘要
背景:血管紧张素转换酶作为肾素-血管紧张素系统的关键酶,通过影响P物质降解等机制参与阿尔茨海默病的发生与发展。目的:分析血管紧张素转换酶基因多态性与阿尔茨海默病的关系,探讨性别、高血压病对两者关系的影响。方法:纳入96例阿尔茨海默病患者,102例年龄、性别等具有可比性的正常人作为对照组。采集血液,抗凝后进行DNA抽提,用PCR反应进行扩增,20 g/L琼脂糖凝胶电泳,紫外光下观察结果,分别计算II、DD型纯合子及ID杂合子基因型及基因型频率。在此基础上,综合分析收集的相关临床资料,进行性别及血压分层分析。结果与结论:阿尔茨海默病组与对照组血管紧张素转换酶基因型和等位基因频率差异有显著性意义,伴有高血压的阿尔茨海默病患者与对照组基因型和等位基因频率差异有显著性意义;不同性别分层及血压正常的阿尔茨海默病组与对照组基因型和等位基因频率差异无显著性意义。提示血管紧张素转换酶基因多态性与阿尔茨海默病的发病有关,其中II基因型可能是阿尔茨海默病发病的危险因素,血管紧张素转换酶基因型可能是阿尔茨海默病伴高血压病的危险因素。
BACKGROUND： Angiotensin-converting enzyme as a key enzyme of the renin-angiotensin system, through the degradation effects of substance P mechanism, is involved in the occurrence and development of Alzheimer＇s disease.
OBJECTIVE： To research the relationship between angiotensin-converting enzyme gene polymorphism and Alzheimer＇s disease in Jiamusi region, as well as the effect of gender and hypertension on the relationship.
METHODS： This case-control study included 96 Alzheimer＇s disease patients. Another 102 subjects served as controls coming from the same area and in the same environmental condition. DNA segments were amplified using PCR in 20 g/L agarose gel electrophoresis and observed under ultraviolet lamp. II, ID, DD genotypes and genotype frequencies were calculated for statistical analysis. On this basis, according to clinical data collected, we investigated association of Alzheimer＇s disease with hypertension and gender.
RESULTS AND CONCLUSION： There was significant difference between Alzheimer＇s disease patients and controls in angiotensin-converting enzyme genotypes and allele frequency. There was statistically significant difference between Alzheimer＇s patients with hypertension and controls in angiotensin-converting enzyme genotypes and allele frequency. There was no statistical difference between Alzheimer＇s disease patients with different genders and controls in angiotensin-converting enzyme genotypes and allele frequency. These findings indicate that there are some relationships between angiotensin-converting enzyme polymorphism and Alzheimer＇s disease. II genotype is a risk factor for Alzheimer＇s disease, angiotensin-converting enzyme II genotype is a risk factor for Alzheimer＇s disease with hypertension.
出处
《中国组织工程研究》
CAS
CSCD
2014年第2期259-264,共6页
Chinese Journal of Tissue Engineering Research
基金
黑龙江省自然科学基金项目(C201242)
黑龙江省教育厅基金面上项目(12511562)~~
