摘要
目的分析胃癌患者与正常人外周血中及胃癌患者不同类型组织中的肥大细胞的分布情况;研究肥大细胞在胃癌微环境中的免疫抑制表型及对肿瘤细胞促炎的抑制作用。方法采用流式细胞术检测,分析56例患者的外周血、肿瘤组织、癌旁组织、非肿瘤正常组织的肥大细胞占CD45+总白细胞的比例,以及其在各组织中共刺激分子HLA-DR的表达情况;同时采用免疫组织化学染色技术针对肥大细胞特异性标志胰蛋白酶(tryptase)原位分析其在各组织中的浸润情况;最后通过人肥大细胞系HMC-1与胃癌细胞系BGC-823共培养,探讨肥大细胞抑制肿瘤细胞分泌促炎因子TNF-ɑ的作用。结果正常人和胃癌患者外周血中肥大细胞的比例没有明显差异(P>0.05),胃癌组织内肥大细胞的比例明显高于癌旁组织(P<0.05)和正常组织(P<0.01);相对于癌旁组织和非肿瘤正常组织,胃癌组织中浸润的肥大细胞显著低表达HLA-DR;HMC-1细胞能抑制BGC-823细胞分泌促炎症因子TNF-α。结论肥大细胞在胃癌微环境中特异性浸润增高,且具有一定免疫抑制表型,提示可能具有抑制胃癌肿瘤细胞促炎症作用的功能。
To investigate the immunosuppressive phenotype of mast cells and the immunosuppressive role of mast cells to inhibit the release of pro-inflammmatory factor by tumor cells, we analyze distribution of mast cells in periphery blood and different types of tissues of gastric cancer （GC） patients and normal people. Flow cytometry was employed to detect the ratio of mast cells in CD45＋ leukocytes on mast cells from tissues of 56 GC patients. Specimens of using anti-tryptase antibody to stain mast cells. Finally, we and the expression of co-stimulating molecule HLA-DR GC patients were investigated immunohistochemically, found that mast cell line HMC-1 could inhibit tumor cell to secret pro-inflammatory molecule TNF-α from GC cell line BGC-823; the frequency of mast cells in GC patients and normal people had no significant difference （P 〉 0.05）. But the percentage of mast cells among total CD45＋ cells was significantly higher in tumor tissues than that in peritumoral （P〈 0.05） or non-tumor tissues （/9〈 0.01）. In tumor tissues, infiltrating-mast cells displayed low level of co-stimulating molecule HLA-DR expression. In addition, HMC-1 inhibited BGC-823 to secret pro-inflammatory molecule TNF-α. So, we concluded that mast cells are enriched in GC environment with certain immunosuppression phenotype, and may play an immunosuppressive role on tumor cells.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2014年第1期1-5,共5页
Immunological Journal
关键词
胃癌
肥大细胞
肿瘤细胞
Gastric cancer
Mast cell
Tumor cell
