摘要
目的：了解黄芪（ＡＭ）对人肾小球系膜细胞（ＨＭＣｓ）分泌基质和表达ＣＤ４４ｍＲＮＡ的影响。方法：应用不同浓度 ＡＭ或 ＡＭ血清处理经 ＰＤＧＦ－ＢＢ刺激的 ＨＭＣｓ，分别采用 ＭＴＴ法和 ＥＬＩＳＡ方法检测 ＨＭＣｓ增殖和Ⅳ型胶原（Ｃｏｌ－Ⅳ）分泌，用ＲＴ－ＰＣ Ｒ法检测ＡＭ对ＨＭＣｓ表面ＣＤ４ ４ｍＲＮＡ表达的影响。结果：经ＡＭ或ＡＭ血清处理后，由ＰＤＧＦ－ＢＢ诱导的ＨＭＣｓ增殖与未处理对照组相比呈显著抑制，并呈剂量依赖效应；ＨＭＣｓ分泌Ｃｏｌ-ＡⅣ和ＣＤ４４ｍＲＮＡ表达水平较未处理对照组相比也有显著下降，并与剂量呈一定相关。结论；ＡＭ可抑制ＨＭＣｓ增殖和Ⅳ型胶原蛋白的过度生成，并且这种作用可能部分是通过降低 ＨＭＣｓ表面 ＣＤ４４ ｍＲＮＡ的过表达而介导的。本研究结果提示，ＡＭ对延缓慢性肾脏疾病向肾硬化进展可能有益。
Objective: To investigate the effect of AM on matrix secretion and CD44 mRNA expreSSion in human renal mesangial Pells (HMCh). Methods: Cultured HMCs were treated with AM and AM serum in different concentrations in the presence or absence of PDGF- BB, the proliferation and type Ⅳ collagen secretion (Col - Ⅳ ) in HMCs were measured by MTT assay and ELISA, and expression of CD44 mRNA was estimated by RT - PCR method, respectively. Results: After AM or AM serum treatment, the proliferation of HMCs induced by PDGF - BB was inhibited significantly in a dine - dependent manner compared with untreated control; Col -Ⅳ secretion of HMCs and CD44 mRNA level were significantly decreased in a dose - dependent manner compared with untreated control. Conclusions: AM could inhibit proliferation and matrix over - synthesis of HMCs. And these might be mediated partly by decrease of CD44 mRNA over- expression on the sufface of HMCs. The present report showed that AM could have a beneficial effect on delay that chronic renal diseases progress to glomerulosclerosis.
出处
《中国中西医结合肾病杂志》
2001年第1期13-16,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
上海市卫生局中医处科研基金项目(No.98A302)
