摘要
目的：探讨淋巴细胞功能相关抗原－１（ＬＦＡ－１）对活动性狼疮肾炎（ＬＮ）外周血单个核细胞（ＰＢＭＣ）白介素 １０（ＩＬ－１０）的调节作用。方法：利用半定量 ＲＴ－ＰＣＲ技术观察 ＬＦＡ－ １共刺激对活动性 ＬＮ患者 ＰＢＭＣ ＩＬ－１０ ｍＲＮＡ表达的影响。结果：单独抗ＣＤ３抗体（３０ ｎｇ／ｍｌ）能诱导ＬＮ 患者ＰＢＭＣ ＩＬ－１０ ｍＲＮＡ轻微表达（０．４３＋０．０３ｖｓ ０．５５±０．４４，Ｐ＜０．０５）而在抗ＣＤ３抗体（３０ ｎｇ／ｍｌ）存在的情况下，抗ＬＦＡ－１抗体（２ μｇ／ｍｌ，相当于ＬＦＡ－１配体）共刺激时明显增加了ＰＢＭＣ ＩＬ—１０ ｍＲＮＡ表达（１．３１±０．０８ｖｓ ０．５５＋０，０４，Ｐ＜０．０１）。ＬＦＡ－１中和抗体明显抑制了这种共刺激诱导的ＩＬ—１０ ｍＲＮＡ表达（１．３１±０．０８ｖｓ ０．６４ ±０．０３，Ｐ＜０．０１）。抗ＬＦＡ－１抗体单独刺激时并不引起 ＩＬ－１０ ｍＲＮＡ表达变化（Ｐ＞０．０５）。结论：ＬＦＡ－１作为共刺激分子诱导 ＩＬ－ １０ ｍＲＮＡ表达可能是其参与 ＬＮ发病的机制之一。
Objective: To elucidate the role of leukocyte function - associated antigen - 1 (LFA - 1 ) on the pathogenesis of lupus nephritis(LN). Methods: 14 active LN patients (male to female 1/13, aged 32 ±4. 7)were selected for this study, With RT - PCR techniques, We to observe Interleukin- 10 mRNA expression in peripheral blood mononuclear cell (PBMC)through LFA - 1 co - stimulation in combination with anti - CD3 antibodies. Results: Stimulation with anti - CD3 antibodies alone promoted slightly the expression of InterleukinL - 10 mRNA (P < 0. 05), but stimulation through anti - LFA - 1- bodies in combination with anti - CD3 antibodies elevateds markedly the expression of Interleukin - 10 mRNA (P < 0. 01 ). the expression of Interleukin - 10 mRNA by LFA - 1 co - stimulation in combination with anti - CD3 antibodies was significantly inhibited when neutralizing anti - LFA - 1 bodies was added. Conclusion: LFA- 1 may serve an important role in the pathogenesis of lupus nephritis as a potent molecule by inducing the expression of Interleukin - 10 mRNA on the ligand bound.
出处
《中国中西医结合肾病杂志》
2001年第1期10-12,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
中山医科大学附属第一医院211工程基金资助项目(No.98150)
