摘要
将超声法预处理剩余污泥得到的提取液用于培养高效生物破乳菌Alcaligenes p．S—XJ-1，以探索剩余污泥资源化利用的新途静．使用超声能量（氐）为443～56647k-T／kgTS的超声条件处理剩余污泥，获得的污泥提取液中氨氮、有机氮、总磷的浓度最高分别为171．94，142．20，76．29mg／L，提取液中包含K、Ca、Mg、Fe等破乳菌生长必须的金属元素．将污泥提取液（Es为885～56647kJ／kgTS）作为培养基周于合成高效破乳菌Alcaligenessp．S-XJ1，产量较MMSM培养基可提高0．3％~68．3％，其中B为56647kJ／kgTS时破乳菌产量最高，为2．03g／L．污泥提取液中的pH缓冲能力对菌体产量有重要影响，提取液的有机氮浓度可能是破乳蒲产量提高的关键因素．污泥提取液培养的破乳荫破乳性能稳定保持在80．0％左右，菌体细胞表面疏水性与菌体C／N较MMSM培养基培养菌体无明硅差异．说明以剩余污泥超声提取液作为培养基可以培养稳定高效的破乳菌Alcaligenesso．S-XJ-1．
In order to find a new way of excess sludge utilization, sludge extract was used in the biosynthesis of demusifier by Alcaligenes sp. S-XJ-1. It was practical to cultivate highly efficient demulsifying strains in sludge extract. Sludge extract was obtained by the untrasound condition of 443-56647kJ/kg TS. In sludge culture medium, maximun concentration of ammonia nitrogen, organic nitrogen and phosphorus were 171.94,142.20 and 76.29mg/L. Sludge extract contained metallic elements such as K, Ca, Mg, Fe that were essential to the synthesis of S-XJ-1. After 7days cultivation, the biomass of S-XJ-lin sludge extract was 0.3%-68.3% higher than that in MMSM. Highest yield （2.03g/L） was obtained when specific energy was 56647KJ/kg TS. pH buffering capacity and concentration of organic nitrogen might have a great impact on the yield. The bio-demusifier produced with sludge extract had a quite stable demusifying ration of 80%, of which the CSH and C/N did not show much difference to that with MMSM.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2014年第2期424-430,共7页
China Environmental Science
基金
国家自然科学基金项目(51108333)
国家科技支撑计划(2012BAC11B00)
关键词
剩余污泥
资源化
超声法
生物破乳菌
培养基
excess sludge
utilization
ultrasounication
demulsifyingstrains
culture medium
