摘要
采用抗鸡毒支原体阳性血清包被酶标板作为捕捉抗体 ,以 4株鸡毒支原体特异单克隆抗体作为第二抗体和酶标羊抗鼠IgG作指示抗体建立了一种检测鸡蛋卵黄中鸡毒支原体的双夹心AC_ELISA。该法具有简便、灵敏、快速等优点 ,从采样到获得结果 6小时内完成。检出率高于分离培养法 ,且重复性良好。该方法的建立为鸡群鸡毒支原体感染的检测和净化提供了一种新的可靠方法。
An antigen_capture ELISA(AC_ELISA) was developed for titration of M.gallisepticum in egg yolk.The plates were coated with polyclonal anti_M.gallisepticum serum and reacted with M.gallisepticum antigens,which were detected with species_specific monoclonal antibodies to M.gallisepticum,followed by anti_rat enzyme_labelled conjugate antiserum(IgG).The procedure in detecting the bacteria in egg yolk was accomplished in 6 hours,and showed to be more sensitive than the conventional culture method.Egg yolk samples collected from eggs were tested for M.gallisepticum by the method and showed a positive rate of 62%.The results demonstrated that the AC_ELISA has rapid,sensitive and simple advantages for monitoring chicken flocks and detecting M.gallisepticum in eggs.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2001年第1期50-52,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
天津市教委科技计划项目 !(96 30 7B)
