摘要
将所制备的腹水单抗ＡＢ７、ＢＧ９经硫酸铁盐析、透析，再经ＤＥＡＥ－纤维素柱层析纯化后，采用戊二醛二步法用辣根过氧化物酶标记单抗，建立了用于鉴别传染性法氏囊病病毒抗原型的ＡＣ－ＥＬＩＳＡ方法，并对国内的６个分离株及美国变异株１０８４Ａ进行了检测，结果表明，上述国内分离株均含有ＣＪ８０１株相一致的ＡＢ７、ＢＧ９位点，而１０８４Ａ株缺失ＡＢ７位点。
McAb AB7,BG9 were respectively purified from ascites by ammonium sulfate precipitation and DEAE-cellulose chromatography.The purified McAbs were conjugated to horseradish peroxidase by glutaraldehyde and the antigen-capture enzyme-linked immunosorbent assay (AC-ELISA)was established.Six domestic IBDV isolates and one American variant strain(1084A) were analyzed by AC-ELISA. The results showed that all the domestic isolates shared the epitopes defined by McAb AB7 and BG9 with CJ801strain,the epitopes defined by McAb AB7 was not found on 1084A strain.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1996年第5期443-447,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
