摘要
[目的]优选灰毡毛忍冬的组织培养的最佳培养基.[方法]选用春季萌发的带腋芽的灰毡毛忍冬茎段为外植体,探讨外植体的最佳灭菌时间,并以MS为基本培养基,附加不同激素浓度配比,筛选丛生芽诱导、增殖及生根培养的最佳培养基.[结果]材料的表面灭菌方法为浓度75％酒精浸泡30 s,然后放到0.1％的HgCl2溶液浸泡8～10 min;在此条件下,污染率为20％,成活率可达80％.最佳诱导培养基为：MS＋ 1.5 mg/L 6-BA＋0.1 mg/L NAA,萌发率达100％.诱导不定芽增殖的最佳培养基为：MS＋ 1.0 mg/L 6-BA＋ 0.1mg/LNAA,增殖率为3.5.诱导生根的最佳培养基为：1/2MS＋ 0.8 mg/L NAA,生根率为90％.[结论]该方法筛选了灰毡毛忍冬的组织培养的最佳培养基,为灰毡毛忍冬的快繁技术提供了理论依据.
[Objective] The study was to optimize the optimum medium for tissue culture of Lonicera macranthoides Hand.-Mazz.[Method] The robust buds or stems with axillary buds of L macranthoides in spring were used as explants to explore suitable disposal of sterilization and build rapid propagation.[Result] The results showed that the suitable disposal sterilization of explants was dipped in 70% alcohol for 30s,rinsed with water twice and then dipped in 0.1% HgCl2 for 8-10min.The pollution rate was 20% and the survival percent of explants reached 80%.The best culture medium for induction of adventitious bud was combination of MS,6-BA 1.5 mg/L and NAA 0.1 mg/L.The best successive culture medium for propagation was combination of MS,6-BA 1.0 mg/L and NAA 0.1 mg/L.The suitable root induction culture was 1/2MS with 0.8 mg/L NAA.The rate of root induction reached 90 %.[Conclusion] The study obtained the optimum medium for tissue culture of L japonica,which provides a theoretical basis for its rapid propagation technique.
出处
《安徽农业科学》
CAS
2013年第30期11952-11953,11964,共3页
Journal of Anhui Agricultural Sciences
