摘要
采用ISSR标记技术对金银花不同品种的遗传变异进行了研究。从100个引物中筛选出10个重复性高、扩增效果好的引物，对23个金银花品种的样本进行PCR扩增，共获得174条稳定的谱带，其中多态性条带169条，多态性比率为97．1％，23个金银花品种间的遗传相似系数范围在0．4713～0．1000之间，平均遗传相似数为0．6299，平均基因多样度（He）和Shannon多样性指数分别是0．3793和0．5563，表明品种间的遗传基础较宽及遗传多样性较高。聚类分析（UPGMA）表明：23个供试金银花品种划分为忍冬、美国忍冬、灰毡毛忍冬3大类。可见，利用ISSR标记可有效地分析金银花种质资源的遗传变异，为金银花品种鉴别、分类、种质资源的有效利用提供理论依据。
The genetic variation of different varieties of Lonicerajaponica was analyzed by inter-simple sequence repeats （ISSR）. Ten ISSR primers with high polymorphic and clear bands were filtered and utilized for testing the genetic variance of 23 Lonicerajaponica varieties. The results demonstrate that 174 DNA fragments were amplified, among which 169 DNA bands were polymorphic with the ratio of polymorphism 97.1%, 23 genetic similarity coefficients ranged from 0.471 3 to 0.862 1, with an average of 0.629 9; Nei＇s gene diversity （He） and Shannon＇s information index were 0.379 3 and 0.556 3 at the species level respectively, which proved that the Lonicerajaponica varieties had a wide genetic base and a high genetic diversity; The cluster analysis presented that the 23 varieties were divided into three main groups by using the unweighted pair-group method with arithmetic average （UPGMA） based on ISSR molecular marker data. So, the ISSR markers can be effectively used to evaluate the genetic diversity of honeysuckles, thus providing a theoretical foundation for the identification and classification of honeysuckle cultivars, and for the efficient use of their germplasm resources.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2013年第11期77-82,共6页
Journal of Central South University of Forestry & Technology
基金
国家公益性行业科研专项"金银花
厚朴优良新品种创制及利用技术研究"(201104023)
关键词
金银花
遗传变异
亲缘关系
ISSR分析
Lonicerajaponica
genetic variation
genetic relationship
ISSR analysis
