摘要
目的探讨1,25(OH)2D3干预下大鼠系膜细胞凋亡及凋亡相关蛋白caspase-3表达的影响。方法体外培养大鼠系膜细胞,分成为正常对照组(0 mol/L)和不同浓度的1,25(OH)2D3干预组(10-6、10-8和10-10mol/L),分别在24和48 h后,采用流式细胞仪,检测系膜细胞的凋亡情况;24 h后免疫荧光染色法,检测caspase-3的表达情况并测定各组的荧光强度;RT-PCR法检测caspase-3的mRNA的表达水平。结果与正常对照组比较,1,25(OH)2D3干预组,诱导系膜细胞凋亡,并呈时间和浓度依赖性(P<0.01);免疫荧光染色法及RT-PCR法,在正常对照组系膜细胞中caspase-3有一定表达,随着1,25(OH)2D3干预浓度越大,系膜细胞内caspase-3的表达增多(P<0.01)。结论 1,25(OH)2D3能诱导系膜细胞凋亡,使凋亡相关蛋白caspase-3的表达增多,并成时间和浓度依赖性。
[ Objective ] To investigate the the effect of 1,25-dihydroxyvitamin D3 on apoptosis of rat glomerular mesangial cellls （MC） and apoptosis-related protein caspase-3 expression. [Methods] Rat glomerular mesangial cells were divided into two groups： control group （0 mol/L）, different dosesof 1,25 （OH）2D3 （10^-6 mol/L; 104 mol/L; 10^-10mol/L） for different time （24 h and 48 h）, the effect of MC apoptosiswasmeasured by flow cytometry; After 24 h, caspase-3 expression in MC was measured by fluorescence microscope and the fluorescent intensity was calculated by image analysis system; RT-PCR was used to observe the expression levels of the mRNA of caspase-3. [ Result ] 1,25（OH）2D3 groups werecompared with control group, the apoptosis was promoted significantly. Those changes showed time-dependent and concentration-dependent （P 〈0.01）; Fluorescence microscope and RT-PCR showed control group had the expression of caspase-3 mRNA. Caspase-3 expression increasedwith the concentration of 1,25（OH）zD3 groups in MC （P 〈0.01）. [ Conclusions ] 1,25 （OH）2D3 induces mesangial cell apoptosis. Caspase-3 expression in- creasesin a time-dependent and concentration-dependent.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第34期1-4,共4页
China Journal of Modern Medicine
基金
国家自然基金(No:81160090/H0509)
