摘要
目的采用RP—HPLC测定香茅挥发油中的橙花醛和香叶醛。方法采用水蒸气蒸馏法提取香茅挥发油，色谱柱为PhenomenexLunaC18柱（150mm×4．6mm，5μm），流动相为甲醇-水（68：32），流速0．8mL·min^-1，检测波长237nm，柱温35℃。结果橙花醛和香叶醛分别为0．0533～0．3199、0．0971～0．5823μg与峰面积呈良好的线性关系，平均回收率分别为100．2％、100．6％，RSD分别为2．07％、2．06％（n=9）。结论所用方法简便、快速，重复性好，准确度高，可作为香茅挥发油的质量控制方法。
OBJECTIVE To establish a method for the determination of neral and geranial in the volatile oil from Cymbopogon citra- tus by RP - HPLC. METHODS The volatile oil was extracted by water steam distillation and HPLC analyses were performed on a Phenomenex Luna C18 column （ 150 mm × 4.6 mm,5 μm） with methanol -water （68 ： 32） as mobile phase at a flow rate of 0.8 mL· min^-1. The detection wavelength was set at 237 nm and the column temperature was maintained at 35 ℃. RESULTS There were good linear relationships for neral and geranial within the concentration range of 0. 0533 -0.3199 μg and 0. 0971 -0.5823 μg, respectively. The average recoveries of neraI and geranial were 100.2% and 100.6% with RSD of 2.07% and 2.06% （ n = 9） ,respectively. CONCLUSION The developed method is simple, rapid, reproducible and accurate, which is helpful for the quality control of the volatile oil from the herb of C. citratus.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2014年第1期82-84,共3页
West China Journal of Pharmaceutical Sciences
基金
海南省教育厅高等学校科学研究资助性项目(编号:Hjkj2010-36)
