摘要
目的测定红景天苷壳聚糖纳米粒(SA-NPs)的包封率并研究其体外释药。方法采用UPLC法测定样品中红景天苷的含量,色谱柱为BEH C18柱(50 mm×2.1 mm,1.7μm),柱温30℃,流动相为甲醇-水(15∶85),流速0.6 mL·min-1,检测波长275 nm。采用0.1 mol·L-1HCl超声提取纳米粒中的红景天苷,测定包封率,并考察纳米粒在人工胃液和人工肠液中的释药特性。结果 SA-NPs的平均包封率为70.15%,在人工胃液中的释放速度较快,在人工肠液中的释放速度相对较慢。结论所用方法可用于SA-NPs包封率的测定,制备的纳米粒具有缓释效果。
OBJECTIVE To detect the encapsulation efficiency of salidroside - ehitosan nanoparticles （ SA - NPs） and evaluate drug release of nanoparticles in vitro. METHODS The determination was performed by UPLC with BEH C18 （ 50 mm x 2.1 ram, 1.7 p,m） column at 30 ℃. The mobile phase was methanol - water（ 15 ： 85 ） with flow rate of 0.6 mL- rain - t and UV detection wavelength of 275 nm. Salidroside in SA - NPs was extracted with 0.1 mob L i HC1 using ultrasonic wave for 5 min. In vitro release of SA - NPs in artifi- cial gastric juice and artificial intestinal juice were also evaluated by UPLC method. RESULTS The average encapsulation efficiency of SA - NPs was 70.15%. The release rate of SA - NPs in artificial gastric juice was fast ,while that of SA - NPs in artificial intestinal juice was relatively slow. CONCLUSION The established method can be used for the determination of encapsulation efficiency of SA - NPs and SA - NPs exhibits sustained release character in vitro.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2014年第1期27-29,共3页
West China Journal of Pharmaceutical Sciences
基金
国家自然科学基金青年科学基金项目(81302729)
中央高校基本科研业务费专项项目(10NZYZJ06)
西南民族大学国家级大学生创新创业训练计划项目(201310656008)
