摘要
利用形态学、细胞学及分子标记技术对从普通小麦和农家二棱大麦的杂交后代中选育的矮秆种质系WB7-3进行了鉴定。结果表明:WB7-3田间农艺性状较好,且表现为矮秆;根尖细胞染色体数目为2n=42,花粉母细胞减数分裂中期I(PMC MⅠ)染色体构型为2n=21Ⅱ,基因组原位杂交(GISH)未出现杂交信号;位于大麦2H染色体短臂上的特异STS引物ABC454能在WB7-3中扩增出大麦特征条带,表明WB7-3含有大麦2HS的染色体片段;利用210对位于小麦各条染色体上的SSR引物对其进行扩增结果显示,2DS上4对引物(Xgdm35、Xgdm5、Xgwm261和Xgwm455)在WB7-3中有条带缺失,由此确定该小大麦矮秆材料WB7-3是一个2DL/2HS小片段易位系。
Morphology,cytology and STS,SSR markers were adopted to identify the dwarf line WB7-3 se- lected from the progenies of common wheat cv. 7182 X two-rowed barley. The results showed that WBT-3 grew evenly presenting favorable agronomic traits and its plant height was very low;the chromosome num- ber of WBT-3 in root tip cells was 42 with 21 bivalents observed in most PMC at M I ,and no fluorescence signals appeared in genomic in situ hybridization （GISH） analysis using barley whole genome DNA; but special strip of barley emerged in WBT-3 at STS marker ABC454-one specific STS primer from barley. Meanwhile,the result of amplification using 210 SSR markers from wheat each chromosome was that all strips of wheat emerged in WB7-3 except four markers （Xgdm35 ,Xgdm5 ,Xgwm261 and Xgwm455） which from the short arm of 2D chromosome. It showed that translocation line. WB7-3 was probably a 2DL/2HS small fragment
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第1期21-25,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省科技统筹创新工程重大项目(2011KTZB02-01)
农业部948项目(2013-Z28)
西北农林科技大学唐仲英育种基金
