摘要
目的 观察微小RNA（miRNA）-374a对乳腺癌细胞增殖、迁移能力的作用,以及对下游基因单核细胞趋化蛋白-1（MCP-1）的调控作用.方法 利用质粒构建miRNA-374a过表达和空白对照细胞株;采用逆转录-聚合酶链反应（RT-PCR）方法检测374a-PLL3.7-MDA和EV-PLL3.7-MDA中miRNA-374a的表达,并分析其与乳腺癌细胞增殖、转移、迁移的相关性.结果 细胞计数试剂盒（CCK-8）细胞增殖实验和单克隆形成实验结果显示,374a-PLL3.7-MDA组细胞增殖能力明显低于对照组细胞EV-PLL3.7-MDA.Transwell小室实验结果显示,374a-PLL3.7-MDA细胞小室膜下表面的细胞数明显少于对照组细胞.374a-PLL3.7-MDA组细胞中MCP-1的表达明显降低.结论 miRNA-374b可能通过MCP-1抑制乳腺癌细胞增殖、迁移能力.
Objective To study the effect of microRNA （miRNA）-374a on proliferation and migration of breast cancer cells and regulation of monocyte chemoattractant protein-1 （MCP-1）.Methods Construct miRNA-374a over-expression MDA-MB-231 breast cancer cells,374a-PLL3.7-MDAs and noload plasmid MDA-MB-231 breast cancer cells,EV-PLL3.7-MDAs by slow virus plasmid PLL3.7; Detect the expression of miRNA-374a by reverse transcriptase-polymerase chain reaction （RT-PCR）.Results miRNA-374a can significantly inhibit breast cancer cell proliferation,migration ability.Cell counting kit-8 （CCK-8） cells proliferation assays and monoclonal formed assays show that 374a-PLL3.7-MDAs proliferation was inhibited than EV-PLL3.7-MDA.Transwell chambers Experimental results show that the chamber film surface of 374a-PLL3.7-MDAs was significantly less than EV-PLL3.7-MDAs.Expression of MCP-1 was inhibited in 374a-PLL3.7-MDAs.Conclusion miRNA-374b may inhibit proliferation and migration of breast cancer cells by MCP-1.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第2期292-294,共3页
Chinese Journal of Experimental Surgery
关键词
微小RNA
单核细胞趋化蛋白-1
乳腺癌
增殖
迁移
MicroRNA
Monocyte chemoattractant protein-1
Breast cancer
Proliferation
Migration
