摘要
目的探讨褪黑素(Mel)对人肺腺癌A549细胞增殖的影响及其作用机制。方法以人肺腺癌细胞株A549为研究对象,分别用不同浓度的Mel处理A549细胞,用四甲基偶氮唑蓝(MTT)法检测Mel对A549细胞增殖的影响;光学显微镜下观察A549细胞形态学变化;Western blot分析细胞MAPK信号通路相关蛋白及骨桥蛋白(OPN)表达的变化。结果 Mel对A549细胞有明显的增殖抑制作用,并呈浓度和时间依赖性;高浓度(2.0 mmol/L)Mel处理组A549细胞数量减少,形态由梭形变成不规则形,细胞间间隙明显增大,成单层生长;Western blot检测显示2.0 mmol/L浓度的Mel能显著抑制JNK、p38的磷酸化,而使ERK磷酸化水平增加;同时,该浓度的Mel能明显抑制OPN蛋白的表达。结论Mel能够呈浓度、时间依赖性抑制A549细胞的增殖,调节MAPK信号通路磷酸化水平和抑制OPN的表达可能为其作用路径之一。
To explore the effect of melatonin on the proliferation of human lung adenocarcinoma cells and its mechanism. Methods Regard lung adenocarcinoma cancer cell strains as the research object. The A549 cells were treated with different concentration of melatonin （Mel）. The proliferation of A549 cells was observed by MTF assay. The change of cell morphology was observed by light microscope. The expression of MAPK related pro- tein and OPN was analyzed by Western blot. Results Mel could inhibit the proliferation of A549 cells in a dose- dependent and time-dependent manner. Compared with control group, A549 cells in the group treated with Mel be- came lesser, more irregular, bigger intercellular gap, non-overlapping and monolayer. The Western blot analysis il- lustrated that 2.0mmoL/L Mel could significantly decreased the expression of phosphorylation of JNK, p38 and OPN in A549 cells （ P 〈 0.05 ）, and the expression of phosphorylation of ERK was increased （ P 〈 0.05 ）, compared with control group. Conclusion Mel can inhibit the proliferation of A549 cells in a dose-dependent and time-de- pendent manner, which may be related to decrease the expression of OPN by regulating the phosphorylation level of MAPK signal pathway.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第2期186-190,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81272399)
安徽省自然科学基金(编号:90413116)
关键词
褪黑素
人肺腺癌A549细胞
细胞增殖
信号转导
骨桥蛋白
melatonin
human lung adenocarcinoma A549 cell
cell proliferation
signal transduction
osteopon-tin
