摘要
目的观察大葱提取物对雄性去势大鼠精囊、前列腺及肛提肌脏器指数及病理组织学的影响。方法雄性大鼠60只,取10只作为正常对照,另50只大鼠在戊巴比妥钠麻醉下摘除双侧睾丸,术后次日将大鼠随机分为5组:(1)去势模型组:蒸馏水10 mL/kg;(2)^(4)大葱提取物大、中、小剂量组:给药量分别为40,20及10 g(按大葱计)/kg,灌胃给药,一日1次,连续给药4周;(5)阳性对照组:丙酸睾酮注射液2 mg/kg,皮下注射,每周给药5次(周一至周五每日给药一次),连续4周。给药结束24 h后,将动物称重、处死解剖,取前列腺、精囊及肛提肌称取重量,计算脏器指数,进行组间t检验;并将肛提肌、前列腺及精囊进行病理组织学检查。结果大葱提取物大、中、小剂量组对肛提肌、前列腺及精囊指数无明显影响,与模型对照组比较无统计学意义(P>0.05)。病理组织学检查结果表明,与去势模型组比较,大葱提取物大、中剂量组对去势大鼠肛提肌有改善作用,可见肌细胞体积增大,排列规整,肌细胞呈圆形,间质细胞无增多;对去势大鼠前列腺亦有改善作用,可见局部腺上皮空泡变性范围减少,程度减轻;大葱提取物大剂量组可增加精囊囊腔数量,减轻囊腔上皮胞浆空泡化程度。结论与去势模型组比较,大葱提取物可改善去势大鼠肛提肌及前列腺的组织萎缩,表明大葱有一定的壮阳作用。
Objective To investigate the effects of Allium fistulosum （L.） var.giganteum extracts on the organ indexes and histopathology changes of seminal vesicle, prostate and levator ani in castrated male rats. Methods 10 out of 60 male rats were used as the normal control. The other 50 male rats were castrated and divided into 5 groups. The groups were：（1） the castration model group, given distilled water 10 mL/kg. （2）-（4） the high, middle and low dose group, given 40 g/kg, 20 g/kg and 10 g/kg （crude drug）of Allium fistulosum （L.） var.giganteum extracts once a day for 4 weeks, respectively. （5） the positive group, given testosterone propionate injection （2 mg/kg） 0.2 mL subcutaneously, 5 days a week from Monday to Friday for 4 weeks. Rats of all groups were weighed and sacrificed 24 hours after stopping dosing. The weights of seminal vesicle, prostate and levator ani were messured and the organ indexes were figured out and compared to that of the castration model group using T-test. Finally, histopathology examination of seminal vesicle, prostate and levator ani were carried out. Results 40 g/kg, 20 g/kg and 10 g/kg of Allium fistulosum （L.） var.giganteum have no obvious effects on seminal vesicle, prostate and levator ani indexes（P＆gt;0.05）. 40g/kg and 20g/kg could improve the conditions of prostate and levator ani histologically. 40g/kg could improve the conditions of seminal vesicle histologically. Conclusion Allium fistulosum （L.） var.giganteum extracts could improve the conditions of levator ani, prostate and seminal vesicle histologically. Allium fistulosum （L.） var.giganteum can strengthen yang.
出处
《食品与药品》
CAS
2014年第1期18-21,共4页
Food and Drug
基金
山东省科技发展计划(2011GNC11301)
山东大学科技发展计划(201101074)
关键词
大葱
肛提肌
前列腺
精囊
壮阳
Allium fistulosum （L.） var.giganteum
levator ani
prostate
seminal vesicle
strengthening yang
