摘要
目的 研究Ⅱ型糖尿病患者脂肪组织胰岛素受体底物 1(IRS 1)及含 2个SH2 结构的蛋白酪氨酸磷酸酶 (SH PTP2 )蛋白表达 ,探讨脂肪组织产生胰岛素抵抗的分子机制。方法 用Western杂交检测Ⅱ型糖尿病患者腹部皮下与大网膜脂肪组织IRS 1、SH PTP2 的蛋白表达 ;同时比较患者自身皮下与大网膜内蛋白表达水平。结果 IRS 1蛋白表达 (A值 )在糖尿病患者皮下脂肪 (2 14± 0 6 7)、大网膜内 (3 2 5± 0 70 )均较对照组 [4 33± 0 5 7(P <0 0 0 1)、8 6 5± 2 85 (P <0 0 5 ) ]减少 ;而SH PTP2与对照组比较差异无显著性 (P >0 0 5 )。IRS 1蛋白表达在患者自身皮下脂肪 (1 0 9± 0 13)较大网膜(2 10± 0 2 2 )减少 (P <0 0 5 ) ;而SH PTP2 在皮下 (70 75± 2 18)则较大网膜 (4 3 6 9± 11 0 7)增加 (P <0 0 5 )。结论 Ⅱ型糖尿病患者脂肪组织IRS 1及SH PTP2 蛋白表达的异常改变可能是引起其产生胰岛素抵抗的机制之一 ;Ⅱ型糖尿病患者的腹部皮下脂肪和内脏脂肪一样存在胰岛素抵抗。
Objective To investigate the protein expression of insulin receptor substrate-1 (IRS-1), protein tyrosine phosphatase with two src-homology 2(SH-PTP 2) in adipose tissues of type Ⅱ diabetic patients, and explore molecular mechanisms of insulin resistance from adipose tissues of type Ⅱ diabetic individuals. Metheds The levels of protein expression of IRS-1 and SH-PTP 2 in adipose tissues from type Ⅱ diabetic individuals were measured by Western blot analysis; meanwhile compared the level of protein expression of one′s own abdominal subcutaneous and omentum majus adipose tissue from patients with type Ⅱ diabetes mellitus. Results The expression of IRS-1 protein in abdominal subcutaneous ( A ) 2.14±0.67 and omentum majus 3.25±0.70 adipose tissues from patients with type Ⅱ diabetes mellitus was significantly lower than that of control group[subcutaneous: 4.33±0.57 ( P <0.001), omentum majus:8.65±2.85 ( P <0.05)]. The expression of SH-PTP 2 protein had no difference compared with control group( P >0.05) . The protein expression of IRS-1 was significantly lower in abdominal subcutaneous adipose tissue (1.09±0.13) than that in omentum majus adipose tissue(2.10±0.22) from the type Ⅱ diabetic patients ( P <0.05), but the protein expression of SH-PTP 2 was significantly higher in subcutaneous adipose tissue (70.75±2.18) than that in omentum majus (43.69±11.07, P <0.05). Conclusion The abnormal changes in expression of IRS-1 and SH-PTP 2 protein in adipose tissues of the patients with type Ⅱ diabetic mellitus may be one of the mechanisms of leading to insulin resistance. The abdominal subcutaneous adipose tissue of type Ⅱ diabetic patients ,as visceral adipose tissues , contributes to their insulin resistance.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2000年第11期739-742,共4页
Chinese Journal of Internal Medicine
关键词
胰岛素受体底物
蛋白酪氨酸磷酸酶
Ⅱ型糖尿病
Diabetes mellitus, non-insulin dependent
Insulin receptor substrate-1
Protein tyrosine phosphatase with two src-homology 2
Insulin resistance
