摘要
目的观察体外培养肺癌细胞bcl2表达与机体免疫攻击致癌细胞凋亡的关系。方法把bcl2表达不同的肺癌细胞株与从肺癌组织中分离出来的肿瘤浸润淋巴细胞tumorinfiltratinglymphocyteTIL共同培养,通过JAM试验观察肺癌细胞凋亡的情况。结果体外JAM试验中,随着TIL浓度增加,bcl2阴性的肺癌细胞凋亡也增加,但bcl2阳性者则无变化。结论bcl2表达阳性的肺癌细胞具有抗凋亡特性,能耐受肿瘤浸润淋巴细胞的免疫攻击。
Objective:The current study was designed to investigate the relationship between b cl-2expression and apoptosis of lung cancer cells in vitro.Methods:Lung cancer cell lines with differen t expression of bcl-2were coculture d with tumor infiltrating lymphocyte(TIL)isolated from fresh tumor samples,and JAM test was performed to evaluate apoptosis of cancer cells.Immunohistochemis try and TUNEL assay were used to determine bcl-2expression and apoptosis of tissue sections of lung cancers respective ly.Results:As shown in JAM test,apoptosis incre ased with the elevation of TIL in coculturing assay in bcl-2negative cancer cell lines,but in one of bcl-2positive cell lines it remained stab le.Conclusions:Lung cancer cells with bcl-2expression may antagonize apoptosis,which may account for their immune evasion mechanism.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2001年第2期168-170,共3页
Chinese Journal of Cancer
