摘要
[目的]观察慢病毒介导的CD-TK融合基因转染神经干细胞(NSCs)对C6胶质瘤生长的抑制作用以及疗效。[方法]利用慢病毒介导的大肠杆菌胞嘧啶脱氨酶(CD)和单纯疱疹病毒胸苷激酶(HSV-TK)融合基因转染NSCs,植入C6胶质瘤模型。用流式细胞仪技术检测C6胶质瘤生长凋亡情况。[结果]成功构建CD-TK基因转染NSCs模型。实验组坏死细胞(17.80%)、早期凋亡细胞及晚期凋亡细胞(33.30%)较对照组增加。CDglyTK-NSC+GCV/5-Fc治疗组肿瘤体积为9.36±2.35mm3,小于其他处理组及对照组的肿瘤体积(P<0.05)。[结论]转染NSCs的CD-TK基因对C6胶质瘤细胞有抑制作用,抗肿瘤效果明显,优于单一的自杀基因系统。
[ Purpose] To investigate the inhibition effect and efficacy of neural stem cells modified by CD-TK gene mediated by lentivirus in the treatment of glioma. [Methods] CD-TK gene was in- troduced into NSCs by lentivirus-mediated transfection and implanted into the model of C6 glioma. FCM was used to observe the apoptosis of glioma in vivo. [Results] NSCs/CD-TK model was successfully eonstrueted. Necrotic cells,apoptotic cells in treatment group were significantly higher than those in other groups（P〈0.05）.Tumor volume in CDglyTK-NSC＋GCV/5-Fc group （9.36±2.35mm3）was smaller than that in other groups（P〈0.05）. [Conclusionl NSCs/CD-TK signifi cantly inhibited the growth of C6 cells with a better anti-tumor effieaey than CD-TK suicide gene.
出处
《中国肿瘤》
CAS
2014年第1期63-67,共5页
China Cancer
