摘要
采用免疫组化的方法，选取地塞米松和Ｐ物质分别代表内分泌和神经因素，研究了它们单独作用及共同作用时对Ｌ４、Ｌ５脊神经节和脊髓后角Ⅰ、Ⅱ层ＣＧＲＰ免疫反应性的影响，并用显微图像分析系统进行相对定量分析处理；同时用热板测痛法测量了痛阈的变化。结果表明：①地塞米松尾静脉注射后痛阈升高，Ｌ４、Ｌ５脊神经节和脊髓后角Ⅰ、Ⅱ层ＣＧＲＰ免疫反应减弱，提示地塞米松可能有抑制痛觉信息一级传入的作用；②Ｐ物质鞘内注射后痛阈降低，Ｌ４、Ｌ５脊神经节和脊髓后角Ⅰ、Ⅱ层ＣＧＲＰ免疫反应增强，Ｐ物质可能有促进痛觉信息一级传入的作用；③地塞米松和Ｐ物质共同作用后痛阈有所降低，Ｌ４、Ｌ５脊神经节和脊髓后角Ⅰ、Ⅱ层ＣＧＲＰ免疫反应强度介于二物质单独作用之间，似乎二者的作用发生了“中和”，但免疫反应仍较对照组增强。结果提示：痛觉的一级传入可能受神经－内分泌相互作用的影响。
Dexamethasone (DEX) and substance P (SP), on behalf of endocrine and nervous factor re- spectively, were used alone or combined to immunocytochemically study their influence on the immunoreactivity of calcitonin gene-related peptide (CGRP) in rat L4-L5 dorsal root ganglia (DRG) and the 1aminaeⅠ, Ⅱof L4- L5 spinal cord. The relative quantitative processing was performed by using micro-image analysis system and the change of the pain threshold was measured by the hot-plat test with an analgesimeter. The results showed DEX administration through the tail vein could elevate the pain threshold and inhibit the immunoreactivity of CGRP in rat L4-L5 DRG and the laminaeⅠ,Ⅱ of L4-L5 spinal cord, suggesting that the DEX might suppress the primary afferent transmission of pain; Intrathecal injection of SP could reduce the pain threshold and en- hance the immunoreactivity of CGRP in rat L4-L5 DRG and the laminaeⅠ,Ⅱof L4-L5 spinal cord, suggesting that the SP might facilitate the primary afferent transmission of pain; Combined use of DEX and SP could re- duce the pain threshold to some extent and the immunoreactivity of CGRP was between that of DEX and SP used alone, but the reactivity was stronger than that of the sham-operated group. It was suggested that the pri- mary afferent transmission of pain might be regulated by neuro-endocrine interaction.
出处
《同济医科大学学报》
CSCD
2000年第6期493-496,共4页
Acta Universitatis Medicinae Tongji
基金
湖北省自然科学基金资助项目(No.96B84)
关键词
降钙素基因相关肽
地塞米松
脊神经节
脊髓后角
calcitonin gene-related peptide
dexamethasone
substance P
dorsal root ganglia
spinal dorsal horn
