摘要
目的 :研究缺氧超极化在缺氧海马神经元中的电生理机制 ,以揭示缺氧超极化在脑损伤中的作用。方法 :应用膜片钳制技术的细胞贴附式膜片记录缺氧海马神经元的单通道电流活动 ,经p CLAMP软件进行采样储存数据和数据的分析处理。结果 :缺氧引起钙激活钾通道 (KCa通道 )和ATP敏感性钾通道 (KATP通道 )的激活 ,增加通道的开放概率。结论 :缺氧超极化可能是缺血性脑损伤早期的重要代偿机制。
Objective: The electrophysiological mechanisms underlying hypoxic hyperpolarization of hippocampal neurons were studied to reveal the role of hypoxic hyperpolarization in brain injuries. Methods: Cell-attached configuration of patch clamp technique was used to record the activity of single channel, application of p-CLAMP software for data sampling, storing, and analysing. Results: Ca 2+ - activated potassium channels (K Ca channels) and ATP - sensitive potassium channels (K ATP channels) could be activated by hypoxia which increased their open probability. Conclusion: Hypoxic hyperpolarization may be an adaptive mechanism that can protect neurons against cerebral ischemic injury in early hypoxic stage.
出处
《泸州医学院学报》
2000年第5期351-353,共3页
Journal of Luzhou Medical College
关键词
缺氧超极化
海马神经元
钙激活钾通道
ATP钾通道
K Ca channels
K ATP channels
Hypoxic hyperpolarization
Patch clamp technique
Hippocampal neurons
