摘要
目的:研究米糠植物甾醇对非酒精性脂肪性肝炎(non-alcoholic steatohepatitis,NASH)大鼠的干预治疗作用。方法:实验选用50只健康SD雄性大鼠,随机分为正常对照组、NASH模型组和米糠甾醇低、中、高3个剂量组(65、130、195 mg/kg)。高脂饲料饲喂造模,各实验组同时进行米糠甾醇溶液灌胃干预。13周后观察各组大鼠肝组织病理学特点,测定血清中血脂总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇含量和谷丙转氨酶、谷草转氨酶、一氧化氮合酶和超氧化物歧化酶活性,并比较各组肝脏指数和脾脏指数。结果:肝脏组织切片观察到NASH模型组有明显的肝细胞脂肪变性和炎症聚集浸润,米糠甾醇各治疗组肝组织病变呈现不同程度减轻。NASH模型组血清高密度脂蛋白胆固醇含量、超氧化物歧化酶、一氧化氮合酶、谷丙转氨酶和谷草转氨酶活性与正常对照组相比,均表现出明显异常;米糠甾醇各剂量组与NASH模型组比较,血清谷丙转氨酶显著降低(P<0.001)、谷草转氨酶/谷丙转氨酶比值显著升高(P<0.01);低剂量组血清超氧化物歧化酶(P<0.05)和一氧化氮合酶(P<0.001)显著降低;中剂量组血清高密度脂蛋白胆固醇显著升高(P<0.05)。结论:米糠甾醇对大鼠非酒精性脂肪性肝炎有预防与治疗作用。
Aim： To investigate the effect of rice bran sterol （RBS） on non-alcoholic steatohepatitis （NASH） in Sprague-Dawley （SD） rats caused by feeding high-fat diet for 13 weeks. Methods： Totally 50 male SD rats were randomly assigned to control group, NASH group and three RBS treatment groups at doses of 65, 130 mg/kg and 195 mg/kg. The NASH group and RBS treatment groups were fed with high-fat diet and the RBS groups were administered with RBS by gavage at the same time. Two percent Tween-80 was added to improve the solubility of RBS. After feeding for 13 weeks, serum total cholesterol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C） and the activities of serum glutamic-pyruvic transaminase （ALT）, glutamic-oxalacetic transaminease （AST）, nitric oxide synthetase （NOS） and superoxide dismutase （SOD） were measured, and organ indexes were compared among groups. Results： Severe steatosis in the NASH group was observed through examining pathological slice of the liver. Serum HDL-C, SOD, NOS, ALT and AST levels in the NASH group significantly abnormal when compared with those in the control group. Serum HDL-C level of the RBS middle-dose group was significantly increased （P 〈 0.05） and sertun ALT levels of all RBS treatment groups were decreased （P 〈 0.001）, while AST/ALT ratio was increased when compared with the NASH group （P 〈 0.01）. Serum SOD and NOS activities in the low-dose group were significantly decreased when compared with those in the NASH group. Conclusion： Moderate dosage of rice bran sterol can ameliorate NASH in rats caused by long-term high-fat diet feeding.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第1期224-228,共5页
Food Science
基金
科技部国际科技合作项目(2009DFA32350)
关键词
米糠甾醇
非酒精性脂肪性肝炎
血脂
抗氧化
谷草转氨酶
谷丙转氨酶
肝脏组织切片
rice bran sterol
non-alcoholic steatohepatitis
serum lipids
antioxidation
glutamic-oxalacetic transaminease/ glutamicpyruvic transaminase ratio
liver tissue slice
