摘要
目的 :探讨高压氧与环孢素A对皮肤移植小鼠脾中活性氧、一氧化氮含量的影响。方法 :供鼠BALB/C ,受鼠C57BL/ 6 ,皮肤移植 ,环孢素A组每日腹腔注射CsA 5mg/kg ,高压氧 (HBO)组每日用 99 2 %氧气0 .2 5MPa作用 1 5h ,14d后 ,取脾称重 ,测脾中丙二醛 (MDA)的含量 ,超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH -PX)、过氧化氢酶 (CAT)的活性和一氧化氮 (NO)的含量 ,一氧化氮合酶 (NOS)的活性。结果 :(1)移植组、高压氧组和环孢素A组的MDA含量 ,GSH -PX、CAT的活性均高于对照组 ;环孢素A组GSH -PX、CAT活性低于、SOD的活性高于移植组 ;高压氧组GSH -PX活性低于、CAT和SOD活性高于移植组 (P <0 0 1)。 (2 )移植组的NOS活性及NO含量高于对照组 ;高压氧组的NO含量低于、NOS活性高于移植组 (P <0 0 1) ;环孢素A组的NO含量、NOS活性变化无显著意义。结论 :皮肤移植小鼠脾细胞过氧化增强 ,NO含量及NOS活性增高 ;高压氧与环孢素A对上述二个系统具有一定作用 ,这可能与其抑制排斥反应有关。
AIM: To study effects of hyperbaric oxygen (HBO) and cyclosporin A (CsA) on the contents of active oxygens and nitric oxide (NO) in spleens of skin transplanted mice.METHODS: The donor mice BALB/C and receptor mice C 57BL/6 were tested for skin transplantation. The HBO group mice were treated with 99.2% oxygen under 0.25 MPa for 1.5 hours, while CsA group mice were treated with CsA 0.5 mg·kg -1·d by abdomen injection. After 14 days, the spleen were extracted the contents of malondialdehyde (MDA) and NO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and NO synthases (NOS) were determined.RESULTS: (1) Compared with the control group, the transplantation group, HBO group and CsA group have markedly increased the content of MDA and the activities of GSH-PX and CAT; Compared with the transplantation group, the CsA group have markedly increased activity of SOD and reduced activities of GSH-PX and CAT; the HBO group have markedly reduced the activity of GSH-PX and increased the activities of CAT and SOD (P<0.01). (2) Compared with the control group, the transplantation group have markedly increased the content of NO and the activity of NOS; Compared with the transplantation group, the HBO group have markedly increased the activity of NOS and reduced the content of NO (P<0.01); The content of NO and the activity of NOS in CsA group was not changed significantly.CONCLUSION: In the lymphocytes of the transplantation group, the peroxidation is intensified, and the content of NO and the activity of NOS increased. HBO and CsA may activate the systems of oxidation/antioxidation and NO/NOS in spleen, which may be related to their mechanism of inhibition rejection.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第12期1286-1288,共3页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助!(No .395 70 778)
关键词
皮肤移植
一氧化氮
高压氧
环孢素A
脾活性
Skin transplantation
Nitric oxide
Hyperbaric oxygenation
Cyclosporine
