摘要
为探明加工番茄种质资源间的亲缘关系，利用SSR标记对20份加工番茄品种及育种材料进行了多样性分析。结果表明：从49对SSR引物中筛选出12对扩增稳定、条带清晰且多态性丰富的引物进行分析，共获得43个位点，多态性位点为37个，多态位点比率86％；供试材料间的遗传相似系数介于0．419～1．000之间，说明加工番茄种质资源间存在一定的遗传差异；通过UPGMA法聚类分析，将20份材料分为3大类群，其中亲缘关系较远的不同类群间及亚类间的种质资源可作为杂交育种的亲本。
Simple sequence repeats （SSR） marker was used to analyze genetic diversity of 20 samples of processing tomato and verify the genetic relationship. 43 bands were selected using the 12 primers, which were highly reproducible, clear, and polymorphic and screened out of 49 primers. Among the obtained bands, 37 were polymorphic bands, accounting for 86% of the total bands. The genetic similarity coefficient among the accessions ranged from 0. 419 to 1. 000, which indicated that genetic basis was relatively abundant among these processing tomato resources. And by UPGMA cluster method,the 20 samples of processing tomato were classified into 3 major groups,of which the materials with distant genetic relationship between major groups or sub-major groups might be used as elite germplasms for further genetic improvement.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第1期196-200,共5页
Journal of Plant Genetic Resources
基金
新疆维吾尔自治区自然科学基金项目(2012211B40)
国家星火计划项目(2011GA89001)
新疆维吾尔自治区"十二五"重大专项(201230116)
