摘要
樟树是我国特有的珍贵用材和经济树种,富含多糖多酚、萜类等次生代谢物质,是香精香料、油脂化工和医药等的重要原料树种。本研究采用高通量测序技术(Illumina HiseqTM2000)首次测定了樟树基因组大小,并利用生物信息方法估计樟树杂合率、重复序列情况和GC含量等基因组信息,为全基因组测序策略的选择提供依据。主要结论如下:(1)樟树基因组大小粗略估计为760 Mb左右;(2)樟树基因组有较高的杂合率和一定的重复,杂合率约为0.65%;(3)由于樟树杂合率较高,全基因组鸟枪法策略不适合该基因组测序分析,可尝试使用BAC-to-BAC策略或fosmid策略,有利于樟树基因组的序列拼接和组装。
Cinnamomum camphora L. Presl is a kind of precious wood species and the main no-timber trees of special local product in our country and possess abundant the secondary metabolism, such as polysaccharides,polyphenols,oleicacid, and terpenoid. C. camphora is a metallic element used in many important industries. In this study,the genome size of C. camphora was determined by new-generation sequencing technologies （Illumina niseqTM 2000）. Hybridity percentage, repeats, and GC depth were also estimated by bioinformatics. The main results were showed that the average genome size and hybridity percentage of C. camphora was about 760 Mb and 0.65% , respectively. Moreover,whole-genome shotgun sequencing should not be used to C. camphora genome sequencing,and the BAC-to-BAC or fosmid could be used.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2014年第1期149-152,共4页
Journal of Plant Genetic Resources
基金
"赣鄱英才555工程"领军人才培养计划项目
关键词
基因组大小
樟树
杂合率
GC含量
genome size
Cinnamomum camphora
hybridity percentage
GC depth
