摘要
目的：观察实验性肝纤维化时肝内纤维及血管的三维结构改变以及复方861治疗对其影响。方法：应用人血白蛋白制造免疫损伤性大鼠肝纤维化模型，采用肝组织连续切片电子计算机辅助病理组织三维重建技术，对肝纤维化以及复方861治疗后肝组织的纤维间隔及肝血窦等结构进行三维观察及体积测量。结果：肝纤维化时，肝内纤维沉积，肝血窦结构紊乱。复方861治疗可使纤维降解；肝血窦改建恢复，肝小叶结构趋于正常。结论：病理组织三维重建研究表明，复方861可以阻断及逆转实验性肝纤维化。
Objective: To observe the three-dimensional structure of fibrotic liver tissue and the effect of herbal compound 861 (Cpd861) on it. Methods: Experimental rat liver fibrosis was induced by human albumin. Liver biopsy specimens were stained with Masson trichrome staining and liver fibrosis was divided into 6 stages. Three-dimensional structure of fibrotic and Cpd861 treated liver tissue were observed by computer aided three-dimensional reconstruction with serial slices. Results: The fibrous septa of fibrotic liver were continuous and wide, the sinusoids were sparsely, irregularly distributed and dilated. After treatment with Cpd861, the fibrous septa were much less than that of without treatment. The sinusoids were more regular and arranged in radius to central veins. Conclusion: Observed three-dimensionally, experimental rat liver fibrosis can be inhibited and reversed by herbal compound.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第6期355-357,共3页
Chinese Journal of Hepatology
关键词
肝纤维化
三维重建
复方中药
动物模型
Liver fibrosis
Herbal compound
Three-dimensional reconstruct
