摘要
目的探讨肝豆灵治疗肝豆状核变性(WD)肝纤维化的可能作用机制。方法 75只Wistar大鼠随机分为空白对照组、模型对照组、肝豆灵组、肝豆灵加青霉胺组、青霉胺组,每组15只。除空白对照组外,其余各组复制铜负荷大鼠模型共12周,从第7周开始肝豆灵组给予肝豆灵0.486g/(kg·d),青霉胺组给予青霉胺0.09g/(kg·d),肝豆灵加青霉胺组给予以肝豆灵和青霉胺联合灌胃,剂量同上。各组均每日灌胃1次,共6周。第13周实验结束后,各组大鼠留取血清标本,运用代谢组学技术检测各组肝纤维化小分子代谢标志物的变化。结果肝豆灵能明显提高铜负荷大鼠血浆亮氨酸、异亮氨酸、胆碱、葡萄糖、3-羟基丁酸的含量,能降低糖蛋白、脂质、乳酸盐、丙酮酸盐的含量。结论肝豆灵对铜负荷大鼠小分子代谢物有明显的调节效应,对代谢异常的修复作用可能是治疗WD肝纤维化的作用机理之一。
Objective WD-indueed liver fibrosis. To study the possible mechanism of Gandouling [ tablets for Wilsong disease （WD） ] for Methods Totally 75 Wistar rats were randomized into the blank control group, model control group, Gandouling group, Gandouling combined with Penicillamine group, and Penicillamine group, with 15 in each. The copper-loaded rat models were copied for 12 weeks in all groups except the blank control group. The Gandouling group was gavaged with 0. 486 g/（ kg ~ d） of Gandouling, the Penicillamine group was gavaged wit.h 0.09 g/（ kg ~ d） of Penicillamine, and the Gandouling combined with Penicillamine group was gavaged with 0. 486 g/ （kg- d） of Gandouling and 0. 09g/（kg ~ d） Penicillamine once daily from the 7th week to the 13th week. The serum specimens were taken and the small molecule makers of liver fibrosis were detected with metabolomic techniques. hydroxybutyric Conclusion Results Gandouling could increase the contents of leucine, isoleucine, choline, glucose and 3- acid, and decrease the contents of glycoproteins, lipids, lactate and pyruvate in copper-loaded rats. Gandouling can regulate small molecule metabolites in copper-loaded rats and repair metabolic abnormalities, which maybe one of the mechanisms for WD
出处
《中医杂志》
CSCD
北大核心
2014年第3期232-237,共6页
Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81202692)
安徽高校省级自然科学研究项目资助(KJ2012Z223)
