摘要
目的应用氢谱核磁共振代谢组学(1H-NMR)方法研究2型糖尿病(T2DM)合并脑梗死患者血清中小分子代谢物代谢轮廓的变化,探讨2型糖尿病合并脑梗死的可能发病机制。方法选择T2DM合并脑梗死患者19例、单纯T2DM患者25例及健康对照者29例,利用1H-NMR方法检测血清的代谢轮廓,通过偏最小二乘判别分析方法,鉴别各组别间血清小分子代谢物的区别。结果从血清偏最小二乘判别分析得分图中可以区分3组患者,与T2DM合并脑梗死有关的特征代谢物包括极低密度脂蛋白、低密度脂蛋白、葡萄糖、乳酸、丙酮酸、3-羟基丁酸、N-乙酰糖蛋白、亮氨酸、缬氨酸、前列腺素D2、前列腺素E2、同型半胱氨酸、氧化三甲胺、甜菜碱等。结论脂类代谢、糖代谢、氨基酸代谢的紊乱可能在T2DM合并脑梗死的发病中起到重要作用。炎症因子、同型半胱氨酸的增加,肠道菌群失调引起的氧化三甲胺、甜菜碱的浓度改变可能也与T2DM合并脑梗死的发生有一定关系。
Aim To investigate the possible mechanism of cerebral infarction in type 2 diabetes mellitus （T2DM） through identifying the changes of metabolic products in serum by applying the method of I H-nuclear magnetic resonance （1H-NMR）. Methods Nineteen patients with cerebral infarction complication in T2DM, 25 T2DM patients without cerebral infarction and 29 healthy controls were included in our study. The micromolecular substances of each participant in serum were detected by I H-NMR. The metabohc profile of each participant was obtained. The partial least square discriminant analysis （PLS-DA） was used to examine the difference of metabolic profile among groups. Results Based on the analysis of score plots of groups, the T2DM patients with cerebral infarction, T2DM patients without cerebral infarction and the group of healthy controls were clearly distinguished by applying PLS-DA. The characteristic metabolic products of patients with cerebral infarction complication in T2DM included very low density lipoprotein （ VLDL）, low density lipoprotein （ LDL）, glucose, 3-hydroxy butanoic acid, lactate, pyruvic acid, glycoprotein, leucine, valine, prostaglandin D2, E2, homocysteine, trimethylamine oxide （TMAO） and betaine. Conclusions The results of our study suggested that the metabolic disorders of lipids, carbohydrate and amino acid may play essential role in the development of T2DM with cerebral infarction. The elevated level of prostaglandin D2 , E2 and homocysteine might play a role in the occurrence of cerebral infarction in T2DM. The change of TMAO and betaine which were caused by enteric dysbacteriosis, also might be related to the occurrence of cerebral infarction in type 2 diabetes mellitus.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2013年第1期63-68,共6页
Chinese Journal of Arteriosclerosis
关键词
2型糖尿病
代谢组学
氢谱核磁共振
脑梗死
Type 2 Diabetes Mellitus
Metabolism
1 H-Nuclear Magnetic Resonance
Cerebral Infraction
