摘要
目的探讨血管内皮生长因子(VEGF)预防血管成形术后再狭窄的机制。方法使用高脂饲养建立实验性动脉粥样硬化家兔模型。将VEGF作用于健康兔和动脉粥样硬化兔主动脉血管内皮细胞(VEC),测定一氧化氮(NO)、125I-内皮素(ET)、6-酮-前列腺素F1α(6-keto-PGF1α)、组织纤溶酶原激活物(t-PA)、纤溶酶原激活物抑制剂(PAI)等。结果 VEC异常组与VEC正常组比较,ET、PAI和t-PA均显著升高,NO、6-keto-PGF1α、t-PA/PAI均明显降低,差异有统计学意义(P<0.05)。不同质量浓度VEGF组与空白对照组比较,NO、6-keto-PGF1α和PAI均升高,其他指标均降低,差异有统计学意义(P<0.05)。结论动脉粥样硬化伴有VEC分泌功能异常,VEGF可促进健康兔和动脉粥样硬化兔的VEC增殖,并促进NO、PAI等分泌,抑制ET、t-PA,降低t-PA/PAI。
Objective To investigate the mechanism of vascular endothelial growth factor（VEGF） in the prevention of restenosis after angioplast. Methods Rabbit model of atherosclerosis with high-fat diet was established. The VEGF was applied in healthy rabbits and atherosclerotic rabbits aortic endothelial cells （VEC） to determine nitric oxide （NO）, ^125I- endothelin （ET）, 6-keto-prostaglandin F 1 α （6-keto-PGF 1 α）, tissue plasminogen activator （t-PA）, plasminogen activator inhibitor （PAI） and so on. Results Comparing with VEC abnormal group and the VEC normal group, ET, PAl and t-PA were significantly increased, NO, 6- keto-PGF 1 α, t-PA/PAI were reduced prominently, and the difference was statistically significant （P〈0.05）. Comparing different concentrations of VEGF group with the control group, NO, 6-keto-PGF1α and PAI were increased,the other indicators were lower, and the difference had statistical significance （P〈0.05）. Conclusion Atherosclerosis is associated with endothelial dysfunction, VEGF can promote the proliferation of VEC in healthy rabbits and atherosclerotic rabbits, encourage the secretion of NO, PAl and so on, inhibit ET,t-PA and reduce t-PA/PAI.
出处
《现代医药卫生》
2014年第2期178-179,共2页
Journal of Modern Medicine & Health
