摘要
以牦牛乳酸水解酪蛋白为氮源,在单因素试验的基础上,采用响应面设计,对巨大芽孢杆菌产青霉素G酰化酶培养基及发酵条件进行了研究.结果表明:以葡萄糖4.28g/L、牦牛乳酸水解酪蛋白10g/L、苯乙酸18.43g/L、MgCl2·6H2O 0.5g/L、FeCl3·6H2O 0.002g/L为培养基,在三角瓶装液量19.3%、接种量5.6%、温度28℃、时间48h、摇床转速220r/min的条件下,所产青霉素G酰化酶活力为232.05U/L,与未添加牦牛乳酸水解酪蛋白对照组相比提高了42.25%.
Taking hydrolysed yak casein acid as the nitrogen source,on basis of single-factor test,using the response surface design,the medium composition and fermenting conditions of bacillus megaterium pro-ducing penicillin G acylase were studied.The results showed that,the penicillin G acylase activity 232.05 U/Lwas obtained when medium composed of glucose 4.28 g/L,hydrolysed yak casein acid 10 g/L, phenylacetic acid 18.43 g/L,MgCl2·6H2O 0.5 g/L,FeCl3·6H2O 0.002 g/L,under this condition of liq-uid volume 19.3% in eriochrome flask,inoculation 5.6%,fermentation at 28 ℃ and 48 h as well as shaking speed 220 r/min,compared with medium of no hydrolysed yak casein acid,the penicillin G acylase activity increased 42.25%.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2013年第6期140-148,共9页
Journal of Gansu Agricultural University
基金
甘肃省科技重大专项(1102NKDN058)
关键词
巨大芽孢杆菌
发酵
青霉素G酰化酶
工艺优化
Bacillus megaterium
fermentation
penicillin G acylase
process optimization
