摘要
目的:通过检测胃泌素及丙谷胺干预下胃癌MKN45细胞DNMT-1基因表达与E-Cadherin和MMP-2基因甲基化变化的相关性。方法:胃癌细胞株分别在胃泌素和丙谷胺干预下分为空白组、胃泌素组、丙谷胺组和混合组,应用SYBR Green I Q-PCR检测各组DNMT-1基因表达水平;运用RQ-MSP法检测用亚硫酸氢盐修饰后的基因组DNA为模板的各组基因甲基化状态。结果:胃泌素组与空白组相比,DNMT-1表达明显增高,而丙谷胺组则表达明显减低(P<0.05);DNMT-1表达量与E-Cadherin甲基化变化存在明显相关性,而与MMP-2甲基化并无明显相关性。结论:胃泌素可促进DNMT-1表达,DNMT-1可能直接影响E-Cadherin甲基化,而其可能间接影响MMP-2表达模式。
Objective：To study gastrin and its antagonist on the expression of DNMT-1 and MMP-2／E-Cadherin methylation in gastric cancer line MKN45 and evaluate their molecules relationship. Method：Separate gastrin and its antagonist’s effect on gastric cancer cells were divided into four groups：controlled group,gastrin group,proglumid group,and mixed group. SYBR GreenⅠQ-PCR method was used to detect expressions of DNMT-1 in all the cases. Genomic DNA modified by sodium bisulfite was used as a template. RQ-MSP detected the methylation status of the gene promoter of MMP-2／E-cadherin. Result：DNMT-1 expression in gastrin group increased significantly in comparison to those of the controlled group（P〈0.05）. However,DNMT-1 expression in proglumid group decreased significantly in comparison to those of controlled group（P〈0.05）. There was a significant positive correlation between the expression of DNMT-1 and E-Cadherin methylation,but no correlation between MMP-2 methylation and the expression of DNMT-1. Conclusion：Gastrin can promote DNMT-1 expression. The expression of DNMT-1 may directly influence E-Cadherin methylation,yet may indirectly influence the expression pattern of MMP-2.
出处
《中国医学创新》
CAS
2013年第36期1-4,共4页
Medical Innovation of China
基金
江西省卫生厅课题(20093095)
