摘要
目的：研究神经生长因子（NGF）和单唾液酸四己糖神经节苷脂（GM1）联合应用对去细胞异种神经支架修复大鼠坐骨神经陈旧性缺损后神经再生和功能恢复的作用。方法：制作大鼠坐骨神经陈旧性缺损模型，用去细胞异种神经支架进行桥接修复。大鼠术侧小腿三头肌内分别注射NGF＋GM1液或Ns液，每日1次连续2周。于修复术后8或14周，检测计算大鼠坐骨神经运动传导速度（MNCV）恢复率和小腿腓肠肌复合动作电位（CMAP）波幅恢复率；辣根过氧化物酶（HRP）作为逆行神经轴突示踪剂注射于损伤神经，观察脊神经节内神经元HRP标记情况；检测小腿三头肌湿重及腓肠肌纤维平均截面积恢复率；免疫荧光染色等方法观察移植的神经支架内及其近、远端吻合口的神经纤维组织学特点。结果：在修复术后8周和12周的动物中都观察到：NGF＋GM1组动物的MNCV恢复率、CMAP波幅恢复率、L3-L5脊神经节HRP标记细胞数、小腿三头肌湿重恢复率及腓肠肌纤维平均截面积恢复率均优于Ns对照组（P〈0．05）；NGF＋GM，组动物移植神经支架内再生神经纤维更加密集、排列规则整齐，神经支架内Schwann细胞（Sc）大量增殖。结论：结果表明NGF及GM1联合去细胞异种神经支架可成功地修复周围神经陈旧性缺损，促进神经再生和功能恢复。
Objective： To evaluate the effects of co-application of NGF and GMl on nerve regeneration and functional re- covery after repairing the obsolete injured sciatic nerve with xenogeneic acellular nerve scaffold. Methods： The obsolete injured sciatic nerve models were set up by transecting the right sciatic nerve of the adult Sprague-Dawley rats and the models were raised without any treatment within two weeks. After two weeks, the injured sciatic nerve of rats was repaired by xenogeneic acellular nerve scaffold. Then the repaired rats were injected with NGF and GM1 or normal saline （NS） for two weeks respectively. After 8 weeks or 14 weeks, the recovery rate of the motor nerve conduction velocities （MNCV） of sciatic nerve and the compound muscular action potential （CMAP） amplitudes of gastrocnemius in the hind limbs were checked. The horseradish peroxidase （HRP） was injected into injured sciatic nerve for retrograde tracing and the HRP-labeled neurons in dorsal root ganglion （DRG） were observed. The recovery rate of wet weight of triceps surae muscle and the mean cross sectional area of gastrocnemius were calculated. The histological characters of repaired sciatic nerve were observed by immunofluorescent staining. Results： The recovery rate of the MNCV, CMAP amplitudes, the HRP-labeled cell numbers in L3-L5 dorsal root ganglion, the recovery rate of wet weight of triceps surae and mean cross sectional area of gastrocnemius in rats treated with co-application of NGF and GM1 were higher than those in normal saline group （P 〈 0.05） at the same time point. The regenerated nerve fibers arranged more densely and orderly, Schwann cells proliferated in larger quantity in nerve scaffold in rats treated with co-application of NGF and GMI. Conclusion： The co-application of NGF and GM1 can enhance the nerve regeneration and functional recovery after an obsolete injured sciatic nerve was re- paired with xenogeneic acellular nerve scaffold.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2014年第1期86-92,共7页
Chinese Journal of Neuroanatomy
基金
银川市科技发展项目(银财发[2011]265号)
宁夏回族自治区科技支撑项目(宁科计字[2012]17号)
