摘要
目的:研究手机900MHz射频磁场对小鼠皮层神经元动作电位的影响。方法:低发射功率为398.1 mW、频率为900 MHz的射频磁场照射急性分离小鼠脑皮层神经元。采用膜片钳技术,观察对照组和暴露组的Na+通道电流I Na、瞬时外向K+通道电流I A和延迟整流K+通道电流I K。结果:900 MHz磁场暴露对神经元离子通道有明显影响:(1)Na+通道激活电位向超极化方向移动,钠电流峰值增大,激活和失活曲线向超极化方向移动;(2)瞬时外向钾通道的激活过程受到抑制;(3)延迟整流钾电流I K受到抑制,且呈时间依赖性。结论:低功率900 MHz射频磁场暴露影响离子通道的I-V特性和动力学特性,神经元动作电位发放阈值变小,动作电位发放频率变缓,改变了动作电位的形状和时程,影响了神经细胞电传导特性,对脑神经元的生理功能产生了一定的影响。
Objective： To investigate the effect of 900 MHz radio frequency （RF） magnetic field emitted by mobile phone on action potential of mouse cortical neurons. Methods ： Acutely isolated cortical neurons of mice were exposed to 900 MHz and 398.1 mW radio frequency magnetic field. INa （sodium channel current）, IA （transient outward potassium current） and IK （delayed rectifier potassium current） were observed in control and exposed group, using whole-cell patch- clamp technique. Results： The neuronal ion channels were affected following exposure to 900 MHz RF magnetic field： （1） the Na＋ channel activation potential moved in the direction of hyperpolarization, peak sodium current increased, the activation and inactivation curves move in direction of hyperpolarization ; （2） the transient outward potassium channel ac- tivation is inhibited; （3） the delayed rectifier potassium current IK was inhibited in a time-dependent manner. Conclu- sion： Exposure to low power 900 MHz RF magnetic field changed the I-V relationsllip and the dynamic characteristics of ionic channels, decreased the neuronal spiking threshold and frequency, changed the shape and the duration of the action potential and affected the electrical conduction characteristics of nerves cell, producing a certain influence on the physio- logical function of brain neurons.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2014年第1期45-50,共6页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(61201106)
关键词
动作电位
神经传导
射频磁场
I-V特性
动力学特性
action potential
nerve conduction
radio frequency （RF） magnetic field
the I[-V characteristics
dy-namic characteristics
