摘要
目的研究原癌基因Bmi-1和hTERT的表达变化及其与细胞凋亡的关系,探讨它们在乳腺癌发生、发展过程中的作用。方法采用MTT法检测乳腺癌细胞的增殖抑制情况,并确定所选用的药物浓度。RT-PCR、Western Blot检测Bmi-1、hTERT的mRNA及蛋白的表达情况;TUNEL、流式细胞技术检测乳腺癌细胞的凋亡情况。结果 5-FU对乳腺癌细胞有明显抑制作用,各实验组与对照组OD值的差异有统计学意义(P<0.05)。用5-FU干预后,实验组Bmi-1和hTERT的mRNA及蛋白表达水平均降低,细胞凋亡指数增加,与对照组相比差异显著有统计学意义(P<0.05),经统计学分析两者呈正相关(P<0.05),且两者的表达与凋亡呈负相关(P<0.05)。结论 Bmi-1和hTERT的表达下调可促进乳腺癌细胞凋亡。
Objective To study the expression changes of proto-oncogene mi-1 and hTERT, and its relationship with apoptosis and to discuss their roles in the occurrence and development of breast cancer. Methods MTT was used to detect the inhibition of breast cancer cell proliferationand the drug concentrations in the experiment; RT-PCR and Western Blot were used to detect the protein and mRNA expression. TUNEL and Flow cytometry were used to detect the apoptosis situation. Results 5-FU could significantly inhibit the growth of breast cancer cell. The differences of OD value between the groups were statistically significant （P〈0.05）. Under the intervention of 5-Fu, the mRNA and Western Blot expression levels of Bmi-1 and hTERT gradually decreased, and the apoptosis index increased, which exhibited great statistical significance （P〈0.05） compared with the control group. Bmi- 1 and hTERT showed a positive correlation （P 〈 0.05）,and their expression had a negative correlation with the apoptosis through the statistical analysis. Conclusion The down-regulation of Bmi-1 and hTERT expression in breast cancer cells can promote the apoptosis.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2014年第1期61-66,共6页
Chinese Journal of Clinical Anatomy
