摘要
利用果糖在碱性条件下可与Cu2+络合后在紫外区有特定吸收波长的原理,建立Cu2+络合物紫外发测定果糖含量的方法,并采用正交实验设计测定果糖含量的最佳条件。结果经正交试验选择果糖含量测定最佳试剂条件为:0.10%的硫酸铜溶液0.8 mL,0.20%的氢氧化钠溶液2.0 mL。测定波长为237 nm,果糖在0.015 mg/mL^0.050 mg/mL浓度范围内线性关系为A=17.614C-0.095 8,r=0.999 9,平均回收率为99.92%(n=9),RSD为0.58%。
To establish and validate a Ultraviolet （UV） method for determination of fructose. The content of fructose was measured by UV spectrophotometry with orthogonal test carried out because fructose reacted with Cu2＋ in alkaline conditions to form a complex compound which had a specific absorption in the UV spectrophotometer. The results showed the optimum condition for determination of fructose was 0.8 mL 0.10 % CuSO4 solution with 2.0 mL 0.20 % NaOH solution. The wavelength of the complex compound was 237 nm. It showed a good linear relationship in the concentration range of 0.015 mg/mL-0.050 mg/mL （A =17.614C-0.095 8, r=0.999 9）. The average recovery （n=9） was 99.92 %, and RSD was 0.58 %.
出处
《食品研究与开发》
CAS
北大核心
2013年第21期86-89,共4页
Food Research and Development
关键词
紫外法
Cu2+络合物
果糖
正交设计
含量测定
UV spectrophotometry
Cu2＋ complex
fructose
orthogonal test
determination
