摘要
利用PCR方法对采自天津地区某猪场的疑似猪圆环病毒2型（PCV-2）感染的病料进行检测，并确定病料中PCV-2的分子特征。将阳性病料接种无PCV污染的PK-15细胞并传代8次，分离出l株PCV-2毒株，命名为PCV-2TJ-1，同时对其全基因组进行克隆和序列分析。结果表明，该分离株基因组全长1767bp，与我国上海LN-19株和韩国的A4099株的核苷酸同源性最高，均达到99．6％。PCV-2全基因组序列的进化分析表明，分离毒株为PCV-2b基因亚型；对分离毒株与5个不同基因亚型的10个参考株的ORF2和ORF3氨基酸序列进行比较，同源性分别为87．1％～97．4％和93．3％～99．0％。结果表明，在天津地区流行的PCV-2毒株为PCV-2b，为PCV-2的分子流行病学、遗传变异及防控奠定了基础。
Samples suspiciously infected with porcine circovirus type 2 （PCV-2） from Tianjin were detected by using polymerase chain reaction （PCR）. PCV-2-positive samples were inoculated into non-PCV con- tained PK-15 cells and passaged eight times. One strain was isolated, and named PCV-2 TJ-1. The com- plete genome of PCV-2 TJ-1 strain was cloned, sequenced and analyzed. Results showed that the full length of genomic sequence of PCV-2 TJ-1 strain was 1 767 nt and the isolate shared the highest identity （99.6%） with that of China LN-19 strain and Korea A4099 strain. Phylogenetic analyses demonstrated that the isolate was classified into PCV-2b genotype. The ORF2- and ORF3-encoded amino acid sequence comparison of PCV-2 TJ-1 and 5 different genotypes of 10 PCV-2 strains showed that the homologies were 87. 1%-97.4% and 93.3%-99.0%, respectively. In conclusion, PCV-2b group strain was predominant prevailing strain in Tianjin, which may provide some basis for studying the molecular epidemiology, varia- tion and prevention of PCV-2.
出处
《动物医学进展》
CSCD
北大核心
2014年第1期58-63,共6页
Progress In Veterinary Medicine
基金
天津市农业科学院院长基金(13004)
关键词
猪圆环病毒2型
基因组
克隆
序列分析
Porcine circovirus type 2
complete genome
cloning
sequence analysis
