摘要
利用PCR方法,对河南郑州、南阳、焦作等地采集的疑似猪圆环病毒2型(PCV2)感染的病料进行检测。PCR检测为阳性的病料经处理后,接种无PCV污染的PK-15细胞中盲传6代,克隆11株PCV2全基因组并进行序列分析。结果表明,11株PCV2中有10株基因组全长为1 767bp,基因组分型为PCV2b,1株为1 768bp,说明PCV2b是河南省断奶后多系统衰竭综合征(PMWS)发生的主要因素;11个毒株的同源性位于94.9%～100%,与其他毒株的同源性为94.7%～100%;10株基因组为1 767bp的毒株处于一大分支上,遗传进化比较稳定;本试验为PCV2在河南地区的分子流行病学、遗传变异及防治奠定了基础。
Samples suspiciously infected with porcine circovirus type 2（PCV2） were collected from several regions in Henan and detected by PCR.PCV2-positive samples were inoculated into non-PCV contaminated PK-15 cells and passaged six times.The complete genomes of 11 PCV2 strains were cloned,sequenced and analyzed.The results showed that the genomes length of 10 strains was 1 767 bp and of 1 strain was 1 768 bp,indicating PCV2b was the main causative agent of PMWS in Henan.The homologies among the 11 strains were 94.9%-100%,and they shared 94.7%-100% homologies with other PCV2 strains.Phylogenetic tree showed the 10 PCV2b strains were closely related.This work may provide some basis for studying the molecular epidemiology,variation and prevention of PCV2 in Henan.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第10期1429-1434,共6页
Chinese Journal of Veterinary Science
基金
河南省杰出人才创新基金资助项目(0621002100)
