摘要
该实验研究NLRP3基因多态性与中国汉族人群结肠癌之间的相关性。采用TaqMan检测法对中国汉族人群208例结肠癌患者和203名正常对照NLRP3的3个位点(rs4925648、rs10754558、rs10925019)进行关联分析,并使用SPSS软件进行单核苷酸多态性分析,比较病例组和对照组等位基因频率、基因型频率及单倍型的差异。结果发现,NLRP3上三个位点基因频率、基因型频率两组间差异不明显(P>0.05)。rs4925648与rs10925019的LD分析其D’值较大(D’=0.798)。但进一步对两位点的单倍型分析发现其各种组合均无统计学差异。该研究结果提示,NLRP3基因的三个位点与中国汉族人群结肠癌的发生相关性无统计学意义。
The aim was to evaluate the relationship between NLRP3 gene and colorectal cancer （CRC）. The sample set consists of 208 CRC patients and 203 controls. 3 SNPs （rs4925648, rs 10754558, rs 10925019） of NLRP3 gene were tested using Taqman technology. The allele frequency, genotypic frequency and haplotype were analyzed between case group and control group. There were no significant difference of allele frequency and genotypic fre- quency between these two groups （P〉0.05）. The D＇ value of rs4925648 and rs10925019 was large （D＇=0.798）. The haplotype of these two SNPs was negative. In conclusion, there was no significant association between rs4925648, rs10754558, rs10925019 and CRC from Chinese Han population.
出处
《中国细胞生物学学报》
CAS
CSCD
北大核心
2014年第1期81-85,共5页
Chinese Journal of Cell Biology
