摘要
目的:探讨小鼠载脂蛋白E(apoE)基因的组织表达特点。方法:提取小鼠的肝、脑和心等17个组织的RNA,采用RTPCR的方法对apoE基因在上述组织中的表达进行研究。结果:apoE基因在肝组织表达最强,其次是脊髓和脑,肺、肾、心、脾、胸腺、小肠、胃、膀胱、血液、肾上腺、子宫、胰腺和主动脉也有不同程度的表达,但在腹肌中几乎不表达。结论:小鼠的apoE基因组织表达谱与人的apoE基因组织表达谱有较高的一致性,与猪的相比差异也不大。
Objective： To analyze the tissue-specific expression profile of mouse apoE gene. Methods： The RNA was isolated from seventeen tissues of mouse and the method of RT-PCR was performed to study the tissue expression of mouse apoE gene. Results： Compared with the rest of the tissues, the expression level of apoE gene was highest in the liver, secondly in the spinal marrow and brain. There were different ex- pression levels in other tissues such as the lungs, kidneys, heart, spleen, thymus, small intestine, stom- ach, bladders, blood, adrenal glands, uterus, pancreas and aorta, whereas almost no expression in the ab- dominal muscle. Conclusion： The tissue-specific expression in the profile of the mouse apoE gene is similar to those of human and pig.
出处
《广西医科大学学报》
CAS
2013年第6期847-849,共3页
Journal of Guangxi Medical University
基金
广西自然科学基金资助项目(No.2010GXNSFA013155)
